Evidence map›Paper›PMID 25653433›Full record

ArticleJournal of virology2015

Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.

Neera Sriskandarajah, Paola Blanchette, Thomas J Kucharski, Jose G Teodoro, Philip E Branton

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Neera SriskandarajahDepartment of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.
Paola BlanchetteDepartment of Biochemistry, McGill University, Montreal, Québec, Canada.
Thomas J KucharskiDepartment of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.
Jose G TeodoroDepartment of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.ORCID http://orcid.org/0000-0002-3713-6839
Philip E BrantonDepartment of Biochemistry, McGill University, Montreal, Québec, Canada Department of Oncology, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada philip.branton@mcgill.ca.
McGill University Health Centre · CAMcGill University · CA

Funding

Canadian Institutes of Health Research MOP-179122Canadian Institutes of Health Research MOP-93753
6 · The paper itself

Abstract

The adenovirus E4orf4 protein expressed at high levels kills cancer cells but not normal human primary cells. Previous studies suggested that disruption of processes that regulate mitosis may underlie E4orf4 toxicity. Here we have used live imaging to show that E4orf4 induces a slowed defective transit through mitosis, exhibiting a delay or often failure in cytokinesis that may account for an accumulation of G1 tetraploids in the population of dying E4orf4-expressing cells.

Indexed as

Cell Line, TumorFlow CytometryHumansMitosisMolecular ImagingTime-Lapse ImagingViral ProteinsE4orf4 protein, adenovirusViral Proteins

Identifiers

PMID25653433
PMCPMC4442376
OpenAlexW2024255389

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.