Evidence map›Paper›PMID 25653339›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2015

Two-photon brightness of azobenzene photoswitches designed for glutamate receptor optogenetics.

Elizabeth C Carroll, Shai Berlin, Joshua Levitz, Michael A Kienzler, Zhe Yuan, Dorte Madsen, Delmar S Larsen, Ehud Y Isacoff

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Review
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  8. Review
  9. Molecules (Basel, Switzerland) · 2021
    Article
  10. Review
  11. Photoisomerization transition state manipulation by entangled two-photon absorption.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  12. Article
  13. Review
  14. Review
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  20. Reversible silencing of endogenous receptors in intact brain tissue using 2-photon pharmacology.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Elizabeth C CarrollDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
Shai BerlinDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
Joshua LevitzDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
Michael A KienzlerDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
Zhe YuanDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
Dorte MadsenDepartment of Chemistry, University of California, Davis, CA 95616;
Delmar S LarsenDepartment of Chemistry, University of California, Davis, CA 95616;
Ehud Y IsacoffDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720; and Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 ehud@berkeley.edu.

Funding

NDC for the Optical Control of Biological FunctionPN2EY018241 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI ISACOFF, EHUD · 2006 to 2015
$37.5M
Synthesis, Characterization, and Testing of Red-Shifted Glutamate PhotoswitchesF32EY022840 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI KIENZLER, MICHAEL A · 2013 to 2015
$165k
NEI NIH HHS F32 EY022840NEI NIH HHS F32EY022840NEI NIH HHS PN2 EY018241NEI NIH HHS PN2EY018241
6 · The paper itself

Abstract

Mammalian neurotransmitter-gated receptors can be conjugated to photoswitchable tethered ligands (PTLs) to enable photoactivation, or photoantagonism, while preserving normal function at neuronal synapses. "MAG" PTLs for ionotropic and metabotropic glutamate receptors (GluRs) are based on an azobenzene photoswitch that is optimally switched into the liganding state by blue or near-UV light, wavelengths that penetrate poorly into the brain. To facilitate deep-tissue photoactivation with near-infrared light, we measured the efficacy of two-photon (2P) excitation for two MAG molecules using nonlinear spectroscopy. Based on quantitative characterization, we find a recently designed second generation PTL, L-MAG0460, to have a favorable 2P absorbance peak at 850 nm, enabling efficient 2P activation of the GluK2 kainate receptor, LiGluR. We also achieve 2P photoactivation of a metabotropic receptor, LimGluR3, with a new mGluR-specific PTL, D-MAG0460. 2P photoswitching is efficiently achieved using digital holography to shape illumination over single somata of cultured neurons. Simultaneous Ca(2+)-imaging reports on 2P photoswitching in multiple cells with high temporal resolution. The combination of electrophysiology or Ca(2+) imaging with 2P activation by optical wavefront shaping should make second generation PTL-controlled receptors suitable for studies of intact neural circuits.

Indexed as

OptogeneticsPhotonsAnimalsAzo CompoundsCells, CulturedIsomerismNeuronsRatsRats, Sprague-DawleyReceptors, GlutamateSpectrum AnalysisazobenzeneAzo CompoundsReceptors, Glutamateazobenzenemultiphotonoptogeneticspharmacologyphotoswitch

Identifiers

PMID25653339
PMCPMC4343171

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.