ArticleScientific reports2015
Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Vein graft failure: Pathophysiology, detection, prevention and emerging therapeutic strategies.Pharmacological reviews · 2026Review
- Pig Genome Editing for Agriculture: Achievements and Challenges.International journal of molecular sciences · 2025Review
- Current Techniques of Gene Editing in Pigs for Xenotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos.Molecular therapy. Nucleic acids · 2023Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- CRISPR Modeling and Correction of Cardiovascular Disease.Circulation research · 2022Review
- Advances in pig models of human diseases.Animal models and experimental medicine · 2022Review
- Gene editing and its applications in biomedicine.Science China. Life sciences · 2022Review
- Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes.Veterinary medicine and science · 2022Article
- Porcine germline genome engineering.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks.Plant molecular biology · 2021Article
- Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.Animals : an open access journal from MDPI · 2021Article
- Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.BMC research notes · 2021Article
- Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.Scientific reports · 2020Article
- One-Step Homology Mediated CRISPR-Cas Editing in Zygotes for Generating Genome Edited Cattle.The CRISPR journal · 2020Article
- Production of gene-edited pigs harboring orthologous human mutations via double cutting by CRISPR/Cas9 with long single-stranded DNAs as homology-directed repair templates by zygote injection.Transgenic research · 2020Article
- CRISPR in livestock: From editing to printing.Theriogenology · 2020Review
- Review
- Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation.The Journal of reproduction and development · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors.
Funding
Abstract
Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in various somatic tissues and ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and reliable approach for generation of gene-modified pigs.
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