ArticleParasites & vectors2015
Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- A helminth-microbiota metabolic relay promotes disease tolerance.Gut microbes · 2026Article
- Assessing diagnostic, vaccine and therapeutic potential of selectedFood and waterborne parasitology · 2025Review
- Heterologous prime-boost immunization based on a human adenovirus 5 vectored containing Trichinella spiralis Cystatin-like protein elicits protective mucosal immunity in mice.PLoS neglected tropical diseases · 2025Article
- Early diagnosis ofVeterinary world · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- The gut microbiota contributes to changes in the host immune response induced by Trichinella spiralis.PLoS neglected tropical diseases · 2023Article
- Immunoproteomic Analysis ofPathogens (Basel, Switzerland) · 2022Article
- Extracellular vesicles from Trichinella spiralis: Proteomic analysis and protective immunity.PLoS neglected tropical diseases · 2022Article
- Article
- Immunomodulatory and biological properties of helminth-derived small molecules: Potential applications in diagnostics and therapeutics.Frontiers in parasitology · 2022Review
- Vaccines as a Strategy to Control Trichinellosis.Frontiers in microbiology · 2022Review
- Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.PLoS neglected tropical diseases · 2021Article
- Evolutionary Analysis of Cystatins of Early-Emerging Metazoans Reveals a Novel Subtype in Parasitic Cnidarians.Biology · 2021Article
- A Case of Trichinellosis in a 14-Year-Old Male Child at Hawassa University Comprehensive Specialized Hospital, Hawassa, Sidama, Ethiopia.Case reports in infectious diseases · 2021Article
- Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection.PLoS neglected tropical diseases · 2020Article
- A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.PLoS neglected tropical diseases · 2020Article
- Article
- The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein FromFrontiers in immunology · 2019Article
- Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting.Parasites & vectors · 2018Article
- Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.PLoS neglected tropical diseases · 2018Article
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Authors and funding
9 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis), in which muscle larvae invade intestine epithelial cells and then develop into adult worms to breed newborn larvae. Thus, intestinal infective larvae are first exposed to the immune system of the host, and antigens from the worms may be the earliest marker in the diagnosis of trichinellosis and may contribute to vaccine development to prevent Trichinella infections in pigs.
methodsA cDNA library of intestinal infective larvae of T. spiralis at 6 hours post infection (p.i.) was constructed and immunoscreened using serum collected from pigs that were infected with T. spiralis at 26 days p.i. T. spiralis cystatin-like protein (Ts-CLP) gene encoding a 45.9 kDa protein was cloned and expressed in Escherichia coli. The rabbit antisera were generated and used to determine the location of Ts-CLP in the parasite. Transcription levels of Ts-CLP in different developmental stages of T. spiralis were observed by RT-PCR. The potential application of recombinant Ts-CLP in diagnosis against T. spiralis infection was tested by ELISA. The immune protection of recombinant Ts-CLP protein against T. spiralis infection was evaluated in mice.
resultsThirty-three positive clones were selected from cDNA library, among which 20 clones encoded the same novel cystatin-like protein (Ts-CLP). Immunolocalisation and real-time quantitative PCR revealed that native Ts-CLP was localised primarily to β-stichocytes and that the Ts-clp gene was transcribed and expressed in all developmental stages of T. spiralis. The recombinant protein rTs-CLP was recognised by pig antiserum as early as 15 days p.i., and could induce protective immunity in mice, with a 61.21% reduction in the number of muscle larvae.
conclusionsThese data preliminarily suggested that Ts-CLP may play an important role in the early infection of T. spiralis and that recombinant Ts-CLP protein is a candidate antigen for diagnosis and vaccine development in Trichinella infections.
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