ArticleOpen biology2015
Promoting effect of 1,25(OH)2 vitamin D3 in osteogenic differentiation from induced pluripotent stem cells to osteocyte-like cells.
Article in Open biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Induced pluripotent stem cells as a new getaway for bone tissue engineering: A systematic review.Cell proliferation · 2017Pooled it
- Unidirectional porous beta-tricalcium phosphate supports three-dimensional osteogenic differentiation of human iPS cells without surface coating.Bone reports · 2026Article
- Effects of zinc carnosine on bone loss in mice with diabetic osteoporosis.Molecular medicine reports · 2026Article
- Nutritional Deficiencies and Reduced Bone Mineralization in Ulcerative Colitis.Journal of clinical medicine · 2025Review
- Recent Advancements Towards the Use of Vitamin D Isoforms and the Development of Their Synthetic Analogues as New Therapeutics.Biomedicines · 2025Review
- The Importance of Vitamin K and the Combination of Vitamins K and D for Calcium Metabolism and Bone Health: A Review.Nutrients · 2024Review
- Article
- Cellular response of advanced triple cultures of human osteocytes, osteoblasts and osteoclasts to high sulfated hyaluronan (sHA3).Materials today. Bio · 2024Article
- Synergistic effect of metformin and vitamin DRegenerative therapy · 2024Article
- Effects of Hyperlipidemia on Osseointegration of Dental Implants and Its Strategies.Journal of functional biomaterials · 2023Review
- Review
- Carnosine and bone (Review).Molecular medicine reports · 2023Review
- Causal associations between insulin-like growth factor 1 and vitamin D levels: a two-sample bidirectional Mendelian randomization study.Frontiers in nutrition · 2023Article
- Recent advances on small molecules in osteogenic differentiation of stem cells and the underlying signaling pathways.Stem cell research & therapy · 2022Review
- The effect of BMP4, FGF8 and WNT3a on mouse iPS cells differentiating to odontoblast-like cells.Medical molecular morphology · 2022Article
- Osteogenic transdifferentiation of primary human fibroblasts to osteoblast-like cells with human platelet lysate.Scientific reports · 2022Article
- Osteon: Structure, Turnover, and Regeneration.Tissue engineering. Part B, Reviews · 2022Review
- New Translational Trends in Personalized Medicine: Autologous Peripheral Blood Stem Cells and Plasma for COVID-19 Patient.Journal of personalized medicine · 2022Article
- Bone regeneration of induced pluripotent stem cells derived from peripheral blood cells in collagen sponge scaffolds.Journal of applied oral science : revista FOB · 2022Article
- Keratinocytes from Gorlin Syndrome-induced pluripotent stem cells are resistant against UV radiation.Medical molecular morphology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We recently reported a new method to purify the induced pluripotent stem (iPS)-derived osteoprogenitors (iPSop). In this paper, we optimized the procedure and characterized cells at each process step. We observed that 10 days of treatment with FGF-2, IGF-1 and TGF-β (FIT) resulted in early-phase osteoblasts and 14 days of treatment resulted in late-phase osteoblasts. We found that treatment with 1,25(OH)2 vitamin D3 increased expression of osteocalcin and decreased expression of tissue-non-specific alkaline phosphatase and runt-related transcription factor 2 (RUNX2) in iPSop-day14 cells (cells treated with FIT for 14 days). Therefore, iPSop-day14 cells were promoted to mature osteoblasts by 1,25(OH)2 vitamin D3 treatment. In addition, we found that 1,25(OH)2 vitamin D3 treatment for 14 days enhanced not only mineralization but also expression of osteocyte markers, including dentin matrix protein-1 and fibroblast growth factor-23, in iPSop cells. Therefore, 1,25(OH)2 vitamin D3 is a potent promoter of osteoblast-osteocyte transition. The results of this study suggest that it is possible to evaluate both early- and late-phase osteoblasts and to apply cells to drug screening for anabolic drugs that stimulate bone formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.