ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2015
Definition of a pool of epitopes that recapitulates the T cell reactivity against major house dust mite allergens.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Broad immunogenicity of house dust mite proteins contrasts restricted specific IgE and IgG4 associated with allergy.PloS one · 2026Article
- A dominant, pan-DR binding epitope of Der p 1 in house dust mite allergy induces tolerance in HLA-DR4 transgenic mice.Frontiers in immunology · 2025Article
- Identification of differentially recognized T cell epitopes in the spectrum of tuberculosis infection.Nature communications · 2024Article
- Identification of cow milk epitopes to characterize and quantify disease-specific T cells in allergic children.The Journal of allergy and clinical immunology · 2023Article
- Identification and Characterization of CD4Journal of virology · 2020Article
- Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans.Science (New York, N.Y.) · 2020Article
- Epitope prediction and identification- adaptive T cell responses in humans.Seminars in immunology · 2020Review
- Single-cell transcriptomic analysis of allergen-specific T cells in allergy and asthma.Science immunology · 2020Article
- A hypoallergenic peptide mix containing T cell epitopes of the clinically relevant house dust mite allergens.Allergy · 2019Article
- Characterization and epitope identification of the T cell response in non-allergic individuals exposed to mouse allergen.The World Allergy Organization journal · 2019Article
- Development of a novel clustering tool for linear peptide sequences.Immunology · 2018Article
- Determination of a Predictive Cleavage Motif for Eluted Major Histocompatibility Complex Class II Ligands.Frontiers in immunology · 2018Article
- Development of a strategy and computational application to select candidate protein analogues with reduced HLA binding and immunogenicity.Immunology · 2018Article
- Allergen and Epitope Targets of Mouse-Specific T Cell Responses in Allergy and Asthma.Frontiers in immunology · 2018Article
- Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes.PloS one · 2018Article
- Urinary Peptides As a Novel Source of T Cell Allergen Epitopes.Frontiers in immunology · 2018Article
- Predicting HLA CD4 Immunogenicity in Human Populations.Frontiers in immunology · 2018Article
- Pathogenic CD4The Journal of allergy and clinical immunology · 2017Review
- Broad perspectives of allergen specific immunotherapy.Human vaccines & immunotherapeutics · 2017Article
- Immunoproteomic analysis of house dust mite antigens reveals distinct classes of dominant T cell antigens according to function and serological reactivity.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAllergens from house dust mites (HDM) are a common cause of asthma. Der p and Der f from Dermatophagoides sp. are strong immunogens in humans. Allergen extracts are used to study T helper (Th2) cell responses to HDM, which are implicated in the development and regulation of allergic disease.
objectiveTo define an epitope mixture that recapitulates, and might substitute for, HDM extract in terms of detecting and characterizing Th2 cell responses.
methodsPeripheral blood mononuclear cells (PBMC) from 52 HDM allergic and 10 non-allergic individuals were stimulated with HDM extracts and assayed with a set of 178 peptides spanning mite allergens group Der p 1, 2, 23 and Der f group 1 and 2 allergens. A pool of the most dominant T cell epitopes identified in the present study and from published literature was assembled and tested for ex vivo T cell responses. Correlation with HDM-specific IgE titres was examined.
resultsPatterns of T cell reactivity to Der p and Der f - derived peptides revealed a large number of epitopes. Clear patterns of immunodominance were apparent, with HDM allergen group 1 and 2 dominant over group 23. Furthermore, within a given antigen, 6-11 epitopes accounted for the vast majority of responses. Based on these results and published data, a comprehensive dust mite pool (DMP) of epitopes was designed and found to allow detection of ex vivo T cell responses. DMP ex vivo reactivity correlated with HDM-specific IgE titres and was similar to that detected with commonly used HDM extracts. Ex vivo DMP stimulation was associated with a predominant Th2 response in allergic donors, and minor reactivity of T cells producing IFNγ, IL17 and IL10. CONCLUSIONS & CLINICAL RELEVANCE: A detailed map of Der p and Der f antigens defined a pool of epitopes that can be used to detect ex vivo HDM responses.
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