ArticleMalaria journal2015
Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real-time PCR.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 4 of them syntheses that pooled it.
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Who cites it
28 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Prevalence and determinants of zoonotic malaria in the Greater Mekong Subregion (GMS) and Malaysia: systematic review and meta-analysis.Malaria journal · 2025Pooled it
- Prevalence and associated factors of malaria among the displaced population in refugee camps in Africa: a systematic review and meta-analysis.Malaria journal · 2025Pooled it
- Global trend of Plasmodium malariae and Plasmodium ovale spp. malaria infections in the last two decades (2000-2020): a systematic review and meta-analysis.Parasites & vectors · 2021Pooled it
- Malaria distribution and performance of malaria diagnostic methods in Malaysia (1980-2019): a systematic review.Malaria journal · 2020Pooled it
- Head-to-head evaluation of a commercial real-time PCR assay targeting plasmodial species causing human malaria against a composite reference standard.European journal of microbiology & immunology · 2026Article
- PvGAP: Development of a Globally Applicable, Highly Multiplexed Microhaplotype Amplicon Panel for Plasmodium vivax.The Journal of infectious diseases · 2026Article
- Malaria Vectors' Diversity and Seasonality in Sustaining Disease Transmission in an Endemic Area of Faladie, Mali.Tropical medicine and infectious disease · 2026Article
- Optimization of the real-time PCR platform method using high-resolution melting analysis and comparison with sequencing and phylogenetic analysis for developing optimal malaria diagnostic methods.Scientific reports · 2025Article
- Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots.PLoS neglected tropical diseases · 2025Article
- Improving diagnostics and surveillance of malaria among displaced people in Africa.International journal for equity in health · 2025Review
- Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria.The American journal of tropical medicine and hygiene · 2024Article
- Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review.Tropical medicine and infectious disease · 2024Review
- Improved limit of detection for zoonoticmedRxiv : the preprint server for health sciences · 2024Article
- Species abundance and density of malaria vectors in Western Thailand and implications for disease transmission.Current research in parasitology & vector-borne diseases · 2024Article
- Evaluation of the performance of advantage P.f. malaria CardParasites & vectors · 2023Article
- The public health response to a Plasmodium malariae outbreak in Penampang district, Sabah during a COVID-19 movement control order.Malaria journal · 2023Article
- Universal two-dimensional labelled probe-mediated melting curve analysis based on multiplex PCR for rapid typing of Plasmodium in a single closed tube.Microbial biotechnology · 2023Article
- Article
- Non-Human Primate Malaria Infections: A Review on the Epidemiology in Malaysia.International journal of environmental research and public health · 2022Review
- A direct, sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening.Infectious diseases of poverty · 2022Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria is a vector borne-parasitic disease transmitted through the bite of the infective female Anopheles mosquitoes. Five Plasmodium species have been recognized by World Health Organization (WHO) as the causative agents of human malaria. Generally, microscopic examination is the gold standard for routine malaria diagnosis. However, molecular PCR assays in many cases have shown improvement on the sensitivity and specificity over microscopic or other immunochromatographic assays.
methodsThe present study attempts to screen 207 suspected malaria samples from patients seeking treatment in clinics around Sabah state, Malaysia, using two panels of multiplex PCRs, conventional PCR system (PlasmoNex™) and real-time PCR based on hydrolysis probe technology. Discordance results between two PCR assays were further confirmed by sequencing using 18S ssu rRNA species-specific primers.
resultsOf the 207 malaria samples, Plasmodium knowlesi (73.4% vs 72.0%) was the most prevalent species based on two PCR assays, followed by Plasmodium falciparum (15.9% vs 17.9%), and Plasmodium vivax (9.7% vs 7.7%), respectively. Neither Plasmodium malariae nor Plasmodium ovale was detected in this study. Nine discrepant species identification based on both the PCR assays were further confirmed through DNA sequencing. Species-specific real-time PCR only accurately diagnosed 198 of 207 (95.7%) malaria samples up to species level in contrast to PlasmoNex™ assay which had 100% sensitivity and specificity based on sequencing results.
conclusionsMultiplex PCR accelerate the speed in the diagnosis of malaria. The PlasmoNex™ PCR assay seems to be more accurate than real-time PCR in the speciation of all five human malaria parasites. The present study also showed a significant increase of the potential fatal P. knowlesi infection in Sabah state as revealed by molecular PCR assays.
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