ArticleBMC microbiology2015
Transient transformation of Podosphaera xanthii by electroporation of conidia.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Saprotrophic Arachnopeziza Species as New Resources to Study the Obligate Biotrophic Lifestyle of Powdery Mildew Fungi.Molecular ecology resources · 2025Article
- Sugars, Lipids and More: New Insights Into Plant Carbon Sources During Plant-Microbe Interactions.Plant, cell & environment · 2025Review
- Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant.Molecular plant pathology · 2024Article
- Comparing Fungal Sensitivity to Isothiocyanate Products on DifferentPlants (Basel, Switzerland) · 2024Article
- Suppression of Chitin-Triggered Immunity by Plant Fungal Pathogens: A Case Study of the Cucurbit Powdery Mildew FungusJournal of fungi (Basel, Switzerland) · 2023Review
- Gene Mining for Conserved, Non-Annotated Proteins ofJournal of fungi (Basel, Switzerland) · 2021Article
- MFS transporter from Botrytis cinerea provides tolerance to glucosinolate-breakdown products and is required for pathogenicity.Nature communications · 2019Article
- Genetic Transformation of the Filamentous FungusFrontiers in microbiology · 2019Article
- Electroporation of germinated conidia and young mycelium as an efficient transformation system for Acremonium chrysogenum.Folia microbiologica · 2019Article
- Transformation by growth onto agro-infiltrated tissues (TGAT), a simple and efficient alternative for transient transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii.Molecular plant pathology · 2018Article
- Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing.Journal of visualized experiments : JoVE · 2017Article
- Mildew-Omics: How Global Analyses Aid the Understanding of Life and Evolution of Powdery Mildews.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPowdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact that has significantly limited their genetic manipulation. In this work, we report a protocol based on the electroporation of fungal conidia, for the transient transformation of Podosphaera fusca (synonym Podosphaera xanthii), the main causal agent of cucurbit powdery mildew.
resultsTo introduce DNA into P. xanthii conidia, we applied two square-wave pulses of 1.7 kV for 1 ms with an interval of 5 s. We tested these conditions with several plasmids bearing as selective markers hygromycin B resistance (hph), carbendazim resistance (TUB2) or GFP (gfp) under control of endogenous regulatory elements from Aspergillus nidulans, Neurospora crassa or P. xanthii to drive their expression. An in planta selection procedure using the MBC fungicide carbendazim permitted the propagation of transformants onto zucchini cotyledons and avoided the phytotoxicity associated with hygromycin B.
conclusionThis is the first report on the transformation of P. xanthii and the transformation of powdery mildew fungi using electroporation. Although the transformants are transient, this represents a feasible method for the genetic manipulation of this important group of plant pathogens.
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