ArticleJournal of visualized experiments : JoVE2015
In vitro functional characterization of mouse colorectal afferent endings.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Sex differences in zymosan-induced behavioral visceral hypersensitivity and colorectal afferent sensitization.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Piezo2 channels expressed by colon-innervating TRPV1-lineage neurons mediate visceral mechanical hypersensitivity.Neuron · 2023Article
- Targeting Two-Pore-Domain Potassium Channels by Mechanical Stretch Instantaneously Modulates Action Potential Transmission in Mouse Sciatic Nerves.ACS chemical neuroscience · 2021Article
- Optical clearing reveals TNBS-induced morphological changes of VGLUT2-positive nerve fibers in mouse colorectum.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- High-Throughput Functional Characterization of Visceral Afferents by Optical Recordings From Thoracolumbar and Lumbosacral Dorsal Root Ganglia.Frontiers in neuroscience · 2021Article
- Computational Modeling of Mouse Colorectum Capturing Longitudinal and Through-thickness Biomechanical Heterogeneity.Journal of the mechanical behavior of biomedical materials · 2021Article
- The heterogeneous morphology of networked collagen in distal colon and rectum of mice quantified via nonlinear microscopy.Journal of the mechanical behavior of biomedical materials · 2021Article
- Enlightening the frontiers of neurogastroenterology through optogenetics.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Extracellular single-unit recordings from peripheral nerve axons in vitro by a novel multichannel microelectrode array.Sensors and actuators. B, Chemical · 2020Article
- Visceral pain from colon and rectum: the mechanotransduction and biomechanics.Journal of neural transmission (Vienna, Austria : 1996) · 2020Review
- Load-bearing function of the colorectal submucosa and its relevance to visceral nociception elicited by mechanical stretch.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Peripheral GABA receptors regulate colonic afferent excitability and visceral nociception.The Journal of physiology · 2019Article
- Optical recording reveals topological distribution of functionally classified colorectal afferent neurons in intact lumbosacral DRG.Physiological reports · 2019Article
- A review on ultrasonic neuromodulation of the peripheral nervous system: enhanced or suppressed activities?Applied sciences (Basel, Switzerland) · 2019Article
- Differential biomechanical properties of mouse distal colon and rectum innervated by the splanchnic and pelvic afferents.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats.Journal of visualized experiments : JoVE · 2017Article
- Article
- In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments.Journal of visualized experiments : JoVE · 2016Article
- Optogenetic activation of mechanically insensitive afferents in mouse colorectum reveals chemosensitivity.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
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2 authors.
Funding
Abstract
This video demonstrates in detail an in vitro single-fiber electrophysiological recording protocol using a mouse colorectum-nerve preparation. The approach allows unbiased identification and functional characterization of individual colorectal afferents. Extracellular recordings of propagated action potentials (APs) that originate from one or a few afferent (i.e., single-fiber) receptive fields (RFs) in the colorectum are made from teased nerve fiber fascicles. The colorectum is removed with either the pelvic (PN) or lumbar splanchnic (LSN) nerve attached and opened longitudinally. The tissue is placed in a recording chamber, pinned flat and perfused with oxygenated Krebs solution. Focal electrical stimulation is used to locate the colorectal afferent endings, which are further tested by three distinct mechanical stimuli (blunt probing, mucosal stroking and circumferential stretch) to functionally categorize the afferents into five mechanosensitive classes. Endings responding to none of these mechanical stimuli are categorized as mechanically-insensitive afferents (MIAs). Both mechanosensitive and MIAs can be assessed for sensitization (i.e., enhanced response, reduced threshold, and/or acquisition of mechanosensitivity) by localized exposure of RFs to chemicals (e.g., inflammatory soup (IS), capsaicin, adenosine triphosphate (ATP)). We describe the equipment and colorectum-nerve recording preparation, harvest of colorectum with attached PN or LSN, identification of RFs in the colorectum, single-fiber recording from nerve fascicles, and localized application of chemicals to the RF. In addition, challenges of the preparation and application of standardized mechanical stimulation are also discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.