ArticleJournal of visualized experiments : JoVE2015
Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Dissection of Neurochemical Pathways Across Complexity and Scale.Journal of neurochemistry · 2025Review
- FOXP2-immunoreactive corticothalamic neurons in neocortical layers 6a and 6b are tightly regulated by neuromodulatory systems.iScience · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- LongitudinalNeurophotonics · 2023Article
- Muscarinic and Nicotinic Modulation of Neocortical Layer 6A Synaptic Microcircuits Is Cooperative and Cell-Specific.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Unveiling the Synaptic Function and Structure Using Paired Recordings From Synaptically Coupled Neurons.Frontiers in synaptic neuroscience · 2020Review
- Layer 4 of mouse neocortex differs in cell types and circuit organization between sensory areas.Nature communications · 2019Article
- High-yield in vitro recordings from neurons functionally characterized in vivo.Nature protocols · 2018Article
- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method.Journal of visualized experiments : JoVE · 2018Article
- Comprehensive Morpho-Electrotonic Analysis Shows 2 Distinct Classes of L2 and L3 Pyramidal Neurons in Human Temporal Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2017Article
- Adenosine Differentially Modulates Synaptic Transmission of Excitatory and Inhibitory Microcircuits in Layer 4 of Rat Barrel Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2017Article
- Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection.Journal of visualized experiments : JoVE · 2017Article
- Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers.Journal of visualized experiments : JoVE · 2016Article
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3 authors.
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Abstract
The combination of patch clamp recordings from two (or more) synaptically coupled neurons (paired recordings) in acute brain slice preparations with simultaneous intracellular biocytin filling allows a correlated analysis of their structural and functional properties. With this method it is possible to identify and characterize both pre- and postsynaptic neurons by their morphology and electrophysiological response pattern. Paired recordings allow studying the connectivity patterns between these neurons as well as the properties of both chemical and electrical synaptic transmission. Here, we give a step-by-step description of the procedures required to obtain reliable paired recordings together with an optimal recovery of the neuron morphology. We will describe how pairs of neurons connected via chemical synapses or gap junctions are identified in brain slice preparations. We will outline how neurons are reconstructed to obtain their 3D morphology of the dendritic and axonal domain and how synaptic contacts are identified and localized. We will also discuss the caveats and limitations of the paired recording technique, in particular those associated with dendritic and axonal truncations during the preparation of brain slices because these strongly affect connectivity estimates. However, because of the versatility of the paired recording approach it will remain a valuable tool in characterizing different aspects of synaptic transmission at identified neuronal microcircuits in the brain.
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