ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2014
False-positive results after environmental pinworm PCR testing due to Rhabditid nematodes in Corncob bedding.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- Pooled it
- Beyond Comfort: A Review of the Critical Role of Bedding Selection in Rodent Welfare, Microenvironment, and Research Reproducibility.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Review
- The application value and challenges of metagenomic next-generation sequencing in the diagnosis of periprosthetic joint infection after arthroplasty.Frontiers in medicine · 2025Review
- An Epidemiological Investigation and Drug-Resistant Strain Isolation ofAnimals : an open access journal from MDPI · 2022Article
- An Update on the Biologic Effects of Fenbendazole.Comparative medicine · 2022Article
- Standardization of an LNA-based TaqMan assay qPCR analysis for Aspiculuris tetraptera DNA in mouse faeces.BMC microbiology · 2020Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Detection of Lactate Dehydrogenase Elevating Virus in a Mouse Vivarium Using an Exhaust Air Dust Health Monitoring Program.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Comparing Mouse Health Monitoring Between Soiled-bedding Sentinel and Exhaust Air Dust Surveillance Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Evaluation of Anthelmintic Resistance and Exhaust Air Dust PCR as a Diagnostic Tool in Mice Enzootically Infected withJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Procedure for Horizontal Transfer of Patient-Derived Xenograft Tumors to EliminateJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Detection and Elimination ofJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Evaluation of Traditional and Contemporary Methods for DetectingJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Booklice (Liposcelis spp.), Grain Mites (Acarus siro), and Flour Beetles (Tribolium spp.): 'Other Pests' Occasionally Found in Laboratory Animal Facilities.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Influence of Rack Design and Disease Prevalence on Detection of Rodent Pathogens in Exhaust Debris Samples from Individually Ventilated Caging Systems.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Surveillance of a Ventilated Rack System for Corynebacterium bovis by Sampling Exhaust-Air Manifolds.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern rodent colonies are housed in individually ventilated cages to protect the animals from contamination with adventitious pathogens. Standard health monitoring through soiled-bedding sentinels does not always detect infections, especially in the context of low pathogen prevalence. Recently proposed alternatives include analyzing environmental samples from the cages or rack exhaust by PCR to improve the detection of rodent pathogens but optimal sampling strategies have not yet been established for different microorganisms. Although generally very sensitive and specific, these molecular assays are not foolproof and subject to false-positive and -negative results and should always be interpreted cautiously with an overall understanding of the intrinsic controls and all the variables that may affect the results. Here, we report a limited Aspiculuris tetraptera outbreak in a mouse barrier facility that was detected by fecal PCR in sentinels and confirmed by fecal flotation and direct cecal examination of both sentinels and colony animals. The outbreak led to a widespread survey of all facilities for pinworms by using environmental PCR from ventilated rack exhaust plenums. Environmental PCR suggested an unexpected widespread contamination of all ventilated racks holding nonautoclaved cages, but results could not be confirmed in sentinel or colony animals by fecal flotation, cecal and colonic examination, or cage PCR testing. After additional investigation, the unexpected environmental PCR results were confirmed as false-positive findings due to the nonspecificity of the assay, leading to the amplification of rhabditid nematodes, which are not infectious in rodents but which contaminated the corncob bedding.
Indexed as
Identifiers
25650980PMC4253587W344006196What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.