ArticleJournal of visualized experiments : JoVE2015
High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging of human tissue sections towards improving pathology.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- FTIR imaging identifies alterations in lung tissue structure and biochemical composition in human idiopathic pulmonary fibrosis.Scientific reports · 2026Article
- Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement.FEBS letters · 2024Review
- Evaluation of Proteomic and Lipidomic Changes inInternational journal of molecular sciences · 2022Article
- Statistical Considerations and Tools to Improve Histopathologic Protocols with Spectroscopic Imaging.Applied spectroscopy · 2022Article
- Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes.Pathology oncology research : POR · 2022Article
- Design Considerations for Discrete Frequency Infrared Microscopy Systems.Applied spectroscopy · 2021Article
- Mid-Infrared Imaging Is Able to Characterize and Separate Cancer Cell Lines.Pathology oncology research : POR · 2020Article
- Biochemical similarity between cultured chondrocytes andBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Macromolecular Characterization of Swordfish Oocytes by FTIR Imaging Spectroscopy.Scientific reports · 2019Article
- Standardization of complex biologically derived spectrochemical datasets.Nature protocols · 2019Article
- Article
- Infrared Spectroscopic Imaging Advances as an Analytical Technology for Biomedical Sciences.Analytical chemistry · 2018Review
- Appropriate Tensile Mode and Timing of Applying Tension to Promote Tendon Gel Regeneration.Tissue engineering and regenerative medicine · 2017Article
- Emerging Themes in Image Informatics and Molecular Analysis for Digital Pathology.Annual review of biomedical engineering · 2016Review
- Development of a practical spatial-spectral analysis protocol for breast histopathology using Fourier transform infrared spectroscopic imaging.Faraday discussions · 2016Article
- Reliability of Histopathology for the Early Recognition of Fibrosis in Traction Alopecia: Correlation with Clinical Severity.Dermatopathology (Basel, Switzerland)Article
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Authors and funding
9 authors.
Funding
Abstract
High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and composition of biomolecules (e.g., lipids, DNA, glycogen, protein, collagen). In an FT-IR image, every pixel within the image comprises an entire Infrared (IR) spectrum that can give information on the biochemical status of the cells that can then be exploited for cell-type or disease-type classification. In this paper, we show: how to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualize FT-IR images. We then present some applications of FT-IR for pathology using the liver and kidney as examples. FT-IR imaging holds exciting applications in providing a novel route to obtain biochemical information from cells and tissue in an entirely label-free non-perturbing route towards giving new insight into biomolecular changes as part of disease processes. Additionally, this biochemical information can potentially allow for objective and automated analysis of certain aspects of disease diagnosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.