ArticleProceedings of the National Academy of Sciences of the United States of America2015
Tunable control of antibody immobilization using electric field.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Article
- Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Electric Field-Assisted Molecularly Imprinted Polymer-Modified QCM Sensor for Enhanced Detection of Immunoglobulin.ACS omega · 2024Article
- Micro-/nanoscale robotics for chemical and biological sensing.Lab on a chip · 2023Review
- A stable physisorbed layer of packed capture antibodies for high-performance sensing applications.Journal of materials chemistry. C · 2023Article
- Advances in single-molecule junctions as tools for chemical and biochemical analysis.Nature chemistry · 2023Review
- pH-Regulated Strategy and Mechanism of Antibody Orientation on Magnetic Beads for Improving Capture Performance ofFoods (Basel, Switzerland) · 2022Article
- Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?Biosensors · 2022Review
- Ultrafast, sensitive, and portable detection of COVID-19 IgG using flexible organic electrochemical transistors.Science advances · 2021Article
- Single-step label-free nanowell immunoassay accurately quantifies serum stress hormones within minutes.Science advances · 2021Article
- Electrobiofabrication: electrically based fabrication with biologically derived materials.Biofabrication · 2019Review
- Single-molecule detection with a millimetre-sized transistor.Nature communications · 2018Article
- Electric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces.Nature communications · 2018Article
- Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.Methods (San Diego, Calif.) · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The controlled immobilization of proteins on solid-state surfaces can play an important role in enhancing the sensitivity of both affinity-based biosensors and probe-free sensing platforms. Typical methods of controlling the orientation of probe proteins on a sensor surface involve surface chemistry-based techniques. Here, we present a method of tunably controlling the immobilization of proteins on a solid-state surface using electric field. We study the ability to orient molecules by immobilizing IgG molecules in microchannels while applying lateral fields. We use atomic force microscopy to both qualitatively and quantitatively study the orientation of antibodies on glass surfaces. We apply this ability for controlled orientation to enhance the performance of affinity-based assays. As a proof of concept, we use fluorescence detection to indirectly verify the modulation of the orientation of proteins bound to the surface. We studied the interaction of fluorescently tagged anti-IgG with surface immobilized IgG controlled by electric field. Our study demonstrates that the use of electric field can result in more than 100% enhancement in signal-to-noise ratio compared with normal physical adsorption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.