ReviewAngewandte Chemie (International ed. in English)2015
Expanding the enzyme universe: accessing non-natural reactions by mechanism-guided directed evolution.
Review in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed.
- Review
- A binuclear copper enzyme platform for enantioconvergent radical (pseudo)halogenation.bioRxiv : the preprint server for biology · 2026Article
- Promiscuity-Guided Enzyme Evolution via Substrate Multiplexed Screening.Angewandte Chemie (International ed. in English) · 2026Review
- The Expanding Reactivity of Cobamide-Containing Proteins: From Mechanistic Understanding to Non-native Biocatalysis.ACS catalysis · 2026Article
- Asymmetric Hydroamination Using Oxidative Radical Initiation in Flavin Enzymes.Journal of the American Chemical Society · 2026Article
- Evolution and Impact of Imine Reductases (IREDs) Research: A Knowledge Mapping Approach.Molecular biotechnology · 2026Review
- Reprogramming Imine Reductases for Enantioselective Reduction of Electron-Rich Enamides.JACS Au · 2026Article
- Photoenzymatic CspProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Chemoenzymatic synthesis of pentalenolactones via stereoselective Riley oxidation by engineered P450Nature communications · 2026Article
- Experimental Lineage and Computational Analysis of a General Aminoxyl-Based Oxidation Catalyst: Generality from Substrate-Specific Interactions.ACS catalysis · 2025Article
- Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid-Liquid Phase Separation.Bio-protocol · 2025Article
- Design and evolution of artificial enzyme with in-situ biosynthesized non-canonical amino acid.Nature communications · 2025Article
- Article
- Combining biocatalytic and radical retrosynthesis for efficient chemoenzymatic synthesis of natural products.Chemical Society reviews · 2025Review
- Tech-Enhanced Synthesis: Exploring the Synergy between Organic Chemistry and Technology.Journal of the American Chemical Society · 2025Review
- Emergent Mechanisms in Biocatalysis.ACS central science · 2025Review
- Data-driven protease engineering by DNA-recording and epistasis-aware machine learning.Nature communications · 2025Article
- Engineering 2-Deoxy-D-ribose-5-phosphate Aldolase for anti- and syn-Selective Epoxidations of α,β-Unsaturated Aldehydes.Angewandte Chemie (International ed. in English) · 2025Article
- De novo design of porphyrin-containing proteins as efficient and stereoselective catalysts.Science (New York, N.Y.) · 2025Article
- Robust enzyme discovery and engineering with deep learning using CataPro.Nature communications · 2025Article
106 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
High selectivity and exquisite control over the outcome of reactions entice chemists to use biocatalysts in organic synthesis. However, many useful reactions are not accessible because they are not in nature's known repertoire. In this Review, we outline an evolutionary approach to engineering enzymes to catalyze reactions not found in nature. We begin with examples of how nature has discovered new catalytic functions and how such evolutionary progression has been recapitulated in the laboratory starting from extant enzymes. We then examine non-native enzyme activities that have been exploited for chemical synthesis, with an emphasis on reactions that do not have natural counterparts. Non-natural activities can be improved by directed evolution, thus mimicking the process used by nature to create new catalysts. Finally, we describe the discovery of non-native catalytic functions that may provide future opportunities for the expansion of the enzyme universe.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.