ArticleMolecular microbiology2015
Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Article
- Structural determinants of SlpA-mediated phage recognition in Clostridioides difficile.PLoS pathogens · 2025Article
- Analysis of essential genes inJournal of bacteriology · 2025Article
- Cryo-EM structure and polar assembly of the PS2 S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.NPJ biofilms and microbiomes · 2025Article
- Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025Article
- Blackleg: A deadly disease with a hidden cause.Open veterinary journal · 2025Review
- Identification of two glycosyltransferases required for synthesis of membrane glycolipids inmBio · 2025Article
- Identification of two glycosyltransferases required for synthesis of membrane glycolipids inbioRxiv : the preprint server for biology · 2025Article
- Article
- Potent and specific antibiotic combination therapy against Clostridioides difficile.Nature chemical biology · 2024Article
- Article
- Clostridioides difficile Biofilm.Advances in experimental medicine and biology · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Bacterial amylases enable glycogen degradation by the vaginal microbiome.Nature microbiology · 2023Article
- An intact S-layer is advantageous to Clostridioides difficile within the host.PLoS pathogens · 2023Article
- Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration.iScience · 2023Article
- Host Immune Responses to Surface S-Layer Proteins (SLPs) ofMicroorganisms · 2023Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment. We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes. In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall. CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats. A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable. We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition. We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
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