Evidence map›Paper›PMID 25649114›Full record

ArticleAnnals of botany2015

UV-B light contributes directly to the synthesis of chiloglottone floral volatiles.

Ranamalie Amarasinghe, Jacqueline Poldy, Yuki Matsuba, Russell A Barrow, Jan M Hemmi, Eran Pichersky, Rod Peakall

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Geographical equations ofFrontiers in plant science · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ranamalie AmarasingheEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Jacqueline PoldyEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Yuki MatsubaEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Russell A BarrowEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Jan M HemmiEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Eran PicherskyEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia.
Rod PeakallEvolution, Ecology and Genetics, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia, Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA and School of Animal Biology & Oceans Institute, University of Western Australia, Perth, WA 6009, Australia rod.peakall@anu.edu.au.
Australian National University · AUUniversity of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimsAustralian sexually deceptive Chiloglottis orchids attract their specific male wasp pollinators by means of 2,5-dialkylcyclohexane-1,3-diones or 'chiloglottones', representing a newly discovered class of volatiles with unique structures. This study investigated the hypothesis that UV-B light at low intensities is directly required for chiloglottone biosynthesis in Chiloglottis trapeziformis.

methodsChiloglottone production occurs only in specific tissue (the callus) of the labellum. Cut buds and flowers, and whole plants with buds and flowers, sourced from the field, were kept in a growth chamber and interactions between growth stage of the flowers and duration and intensity of UV-B exposure on chiloglottone production were studied. The effects of the protein synthesis inhibitor cycloheximide were also examined. KEY

resultsChiloglottone was not present in buds, but was detected in buds that were manually opened and then exposed to sunlight, or artificial UV-B light for ≥5 min. Spectrophotometry revealed that the sepals and petals blocked UV-B light from reaching the labellum inside the bud. Rates of chiloglottone production increased with developmental stage, increasing exposure time and increasing UV-B irradiance intensity. Cycloheximide did not inhibit the initial production of chiloglottone within 5 min of UV-B exposure. However, inhibition of chiloglottone production by cycloheximide occurred over 2 h of UV-B exposure, indicating a requirement for de novo protein synthesis to sustain chiloglottone production under UV-B.

conclusionsThe sepals and petals of Chiloglottis orchids strongly block UV-B wavelengths of light, preventing chiloglottone production inside the bud. While initiation of chiloglottone biosynthesis requires only UV-B light, sustained chiloglottone biosynthesis requires both UV-B and de novo protein biosynthesis. The internal amounts of chiloglottone in a flower reflect the interplay between developmental stage, duration and intensity of UV-B exposure, de novo protein synthesis, and feedback loops linked to the starting amount of chiloglottone. It is concluded that UV-B light contributes directly to chiloglottone biosynthesis. These findings suggest an entirely new and unexpected biochemical reaction that might also occur in taxa other than these orchids.

Indexed as

Ultraviolet RaysCyclohexanonesCycloheximideFlowersOrchidaceaePlant ProteinsPollinationProtein Synthesis Inhibitors2-ethyl-5-propylcyclohexan-1,3-dioneCyclohexanonesCycloheximidePlant ProteinsProtein Synthesis Inhibitors23-diones5-dialkylcyclohexane-1biochemistryChiloglottis trapeziformischiloglottonefloral semiochemicalfloral volatilesOrchidaceaeorchidspollinationsexual deceptionUV-B

Identifiers

PMID25649114
PMCPMC4343295
OpenAlexW2153341947

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.