ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015
Phenol-soluble modulin α induces G2/M phase transition delay in eukaryotic HeLa cells.
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Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
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- The Human LL-37(17-29) antimicrobial peptide reveals a functional supramolecular structure.Nature communications · 2020Article
- Involvement of caspase-1 in inflammasomes activation and bacterial clearance in S. aureus-infected osteoblast-like MG-63 cells.Cellular microbiology · 2020Article
- Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury.PloS one · 2020Article
- Rck ofFrontiers in cellular and infection microbiology · 2020Article
- Staphylococcus aureus induces DNA damage in host cell.Scientific reports · 2019Article
- Extreme amyloid polymorphism in Staphylococcus aureus virulent PSMα peptides.Nature communications · 2018Article
- Heterogeneous Family of Cyclomodulins: Smart Weapons That Allow Bacteria to Hijack the Eukaryotic Cell Cycle and Promote Infections.Frontiers in cellular and infection microbiology · 2017Review
- Staphylococcus aureus Infection Reduces Nutrition Uptake and Nucleotide Biosynthesis in a Human Airway Epithelial Cell Line.Metabolites · 2016Article
- Staphylococcus aureus Phenol-Soluble Modulins Impair Interleukin Expression in Bovine Mammary Epithelial Cells.Infection and immunity · 2016Article
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- Phenol-Soluble Modulins Contribute to Early Sepsis Dissemination Not Late Local USA300-Osteomyelitis Severity in Rabbits.PloS one · 2016Article
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Authors and funding
17 authors at 8 institutions in 6 countries.
Martine Deplanche*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Rachid Aref El-Aouar Filho*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Ludmila Alekseeva*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Emilie Ladier*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Julien Jardin*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Gwénaële Henry*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Vasco Azevedo*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Anderson Miyoshi*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Laetitia Beraud*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Frederic Laurent*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Gerard Lina*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
François Vandenesch*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Jean-Paul Steghens*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Yves Le Loir*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Michael Otto*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Friedrich Götz*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany.
Nadia Berkova*Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1253 STLO, Rennes, France; Agrocampus Ouest, Unité Mixtes de Recherche1253 STLO, Rennes, France; Instituto de Ciências Biológicas- Universidade Federal de Minas Gerais , Belo Horizonte- Minas Gerais, Brazil; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Centre International de Recherche en Infectiologie, Institut National de la Santé et de la Recherche Médicale U1111, Centre National de la Recherche Scientifique Unité Mixtes de Recherche5308, Université Lyon 1, Lyon, France; Hospices Civil de Lyon, Lyon, France; Laboratory of Human Bacterial Pathogenesis, National Institutes of Health, Bethesda, Maryland, USA; and **Microbial Genetics, University of Tübingen, Tübingen, Germany nadia.berkova@rennes.inra.fr.
Institut National de la Recherche Agronomique · MAInstitut Agro Rennes-Angers · FRUniversidade Federal de Minas Gerais · BRUniversité Claude Bernard Lyon 1 · FRCentre International de Recherche en Infectiologie · FRHospices Civils de Lyon · FRNational Institutes of Health · USUniversity of Tübingen · DE
Funding
Staphylococcal pathogenesisZIAAI000904 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI OTTO, MICHAEL · 2009 to 2025
$22.6MIntramural NIH HHS
Abstract
Staphylococcus aureus is a gram-positive bacterium responsible for a wide range of infections. Host cell cycle alteration is a sophisticated mechanism used by pathogens to hijack the defense functions of host cells. We previously demonstrated that S. aureus MW2 (USA400) bacteria induced a G2/M phase transition delay in HeLa cells. We demonstrate here that this activity is triggered by culture supernatant compounds. Using size exclusion chromatography of the MW2 supernatant, followed by mass spectroscopy analysis of corresponding peaks, we identified phenol-soluble modulin α (PSMα) peptides as the likely candidates for this effect. Indeed, synthetic PSMα1 and PSMα3 caused a G2/M phase transition delay. The implication of PSMα in cell cycle alteration was confirmed by comparison of S. aureus Los Angeles County clone (LAC) wild-type with the isogenic mutant LAC∆psmα, which lacks the psmα operon encoding PSMα1-4. PSMα-induced G2/M transition delay correlated with a decrease in the defensin genes expression suggesting a diminution of antibacterial functions of epithelial cells. By testing the supernatant of S. aureus human clinical isolates, we found that the degree of G2/M phase transition delay correlated with PSMα1 production. We show that PSMs secreted by S. aureus alter the host cell cycle, revealing a newly identified mechanism for fostering an infection.
Indexed as
Bacterial ToxinsBlotting, WesternCell ProliferationCells, CulturedCulture Media, ConditionedFlow CytometryG2 Phase Cell Cycle CheckpointsHeLa CellsHumansM Phase Cell Cycle CheckpointsPeptide FragmentsPhenolStaphylococcal InfectionsStaphylococcus aureusTandem Mass SpectrometryBacterial ToxinsCulture Media, ConditionedPeptide FragmentsPhenolcell cycle alterationdefensinsinnate immune responseStaphylococcus aureustoxin
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