ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2015
Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Integrated phenotyping reveals coordinated physiological responses to cold, freezing, and recovery inAoB PLANTS · 2026Article
- Recovery Dynamics of Photosynthetic Performance and Antioxidant Defense in Resurrection PlantsPlants (Basel, Switzerland) · 2025Article
- Two Decades of Desiccation Biology: A Systematic Review of the Best Studied Angiosperm Resurrection Plants.Plants (Basel, Switzerland) · 2021Review
- DNA barcoding and genetic fidelity assessment of micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Activity of δ-aminolevulinic acid dehydratase at Ramonda nathaliae and Ramonda serbica plants during dehydration and rehydration.Biologia futura · 2019Article
- A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
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Authors and funding
4 authors.
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Abstract
Ramonda serbica and Ramonda nathaliae are rare and endemo relict plant species from Balkan Peninsula. An efficient micro propagation and in vitro conservation method via direct and indirect organogenesis from seed and leaf explants, respectively, was established in this study. The seed of both Ramonda species were collected from different populations in Kosovo, and were germinated in nutrient media JG-B without any phytohormone. The highest number of shoots and multiplication rate was observed on JG-B medium supplemented with BAP and IAA (0.5 mg l(-1) each), whereas the highest number of leaves per plantlets was found on WPM and RA medium supplemented with BAP and IAA (0.1 mg l(-1) each). During this stage of micro propagation some significant differences were observed in plantlets from different populations. The indirect organogenesis from parts of leaves of natural plants was not successful due to unavailability of established protocol for disinfections of the plant material. On other hand, parts of leaves from micro propagated plantlets, cultured on MS medium supplemented with different ratio of BAP and NAA, resulted in the highest efficiency for shoot regeneration. In vitro conservation of micro propagated plants at the lower temperature (4 °C) had a significantly positive effect for storage of more than 12 months.
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