ArticleScientific reports2015
Co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 108 citations in OpenAlex.
- Enhancing camptothecin biosynthesis in Camptotheca acuminata through CrLAMT transfer from Catharanthus roseus.Biotechnology letters · 2026Article
- The genome of the medicinal plant Uncaria rhynchophylla provides new insights into monoterpenoid indole alkaloid metabolism and its molecular regulatory mechanism.Molecular horticulture · 2026Article
- Cloning and functional verification ofPeerJ · 2025Article
- A genome assembly of decaploidHorticulture research · 2024Article
- Biotechnological approaches for the production of camptothecin.Applied microbiology and biotechnology · 2024Review
- Exploring the influence of organ differentiation on biosynthesis and accumulation of camptothecin in3 Biotech · 2024Article
- Genome-Wide Mining of CULLIN E3 Ubiquitin Ligase Genes fromPlants (Basel, Switzerland) · 2024Article
- Genome-wide identification of GATA transcription factor family and the effect of different light quality on the accumulation of terpenoid indole alkaloids in Uncaria rhynchophylla.Plant molecular biology · 2024Article
- Review
- Genome-scale metabolic model led engineering ofFrontiers in plant science · 2023Article
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Transcriptome revealing the dual regulatory mechanism of ethylene on the rhynchophylline and isorhynchophylline in Uncaria rhynchophylla.Journal of plant research · 2022Article
- Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila.BMC genomics · 2022Article
- Identification of WRKY transcription factors involved in regulating the biosynthesis of the anti-cancer drug camptothecin inHorticulture research · 2022Article
- Article
- The transcription factor OpWRKY2 positively regulates the biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila.Horticulture research · 2021Article
- Effects of Concentration and Temperature of Nutrient Solution on Growth and Camptothecin Accumulation ofPlants (Basel, Switzerland) · 2020Article
- Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from in Vitro Plant Cultures.Plants (Basel, Switzerland) · 2020Review
- Boosting the Synthesis of Pharmaceutically Active Abietane Diterpenes inFrontiers in plant science · 2020Article
- Transcription FactorInternational journal of molecular sciences · 2019Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Camptothecin (CPT) belongs to a group of monoterpenoidindole alkaloids (TIAs) and its derivatives such as irinothecan and topothecan have been widely used worldwide for the treatment of cancer, giving rise to rapidly increasing market demands. Genes from Catharanthus roseus encoding strictosidine synthase (STR) and geraniol 10-hydroxylase (G10H), were separately and simultaneously introduced into Ophiorrhiza pumila hairy roots. Overexpression of individual G10H (G lines) significantly improved CPT production with respect to non-transgenic hairy root cultures (NC line) and single STR overexpressing lines (S lines), indicating that G10H plays a more important role in stimulating CPT accumulation than STR in O. pumila. Furthermore, co-overexpression of G10H and STR genes (SG Lines) caused a 56% increase on the yields of CPT compared to NC line and single gene transgenic lines, showed that simultaneous introduction of G10H and STR can produce a synergistic effect on CPT biosynthesis in O. pumila. The MTT assay results indicated that CPT extracted from different lines showed similar anti-tumor activity, suggesting that transgenic O. pumila hairy root lines could be an alternative approach to obtain CPT. To our knowledge, this is the first report on the enhancement of CPT production in O. pumila employing a metabolic engineering strategy.
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