ArticleNanotechnology2015
DNA stretching and optimization of nucleobase recognition in enzymatic nanopore sequencing.
Article in Nanotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Direct RNA sequencing in plants: Practical applications and future perspectives.Plant communications · 2024Review
- Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules Using a Biological Nanopore.Analytical chemistry · 2023Article
- Free-energy landscapes of membrane co-translocational protein unfolding.Communications biology · 2020Article
- FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution.Nature communications · 2019Article
- Recent progress in dissecting molecular recognition by DNA polymerases with non-native substrates.Current opinion in chemical biology · 2017Review
- Single-molecule nanopore enzymology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Nanoscale Investigation of Generation 1 PAMAM Dendrimers Interaction with a Protein Nanopore.Scientific reports · 2017Article
- Charge, Diffusion, and Current Fluctuations of Single-Stranded DNA Trapped in an MspA Nanopore.Biophysical journal · 2017Article
- Investigating asymmetric salt profiles for nanopore DNA sequencing with biological porin MspA.PloS one · 2017Article
- Engineering a nanopore with co-chaperonin function.Science advances · 2015Article
- Nanopore Sequencing: Forcing Improved Resolution.Biophysical journal · 2015Article
- Thermal Motion of DNA in an MspA Pore.Biophysical journal · 2015Article
- Unfolding Kinetics of the Human Telomere i-Motif Under a 10 pN Force Imposed by the α-Hemolysin Nanopore Identify Transient Folded-State Lifetimes at Physiological pH.Journal of the American Chemical Society · 2015Article
- DNA-functionalized silicon nitride nanopores for sequence-specific recognition of DNA biosensor.Nanoscale research letters · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
In nanopore sequencing, where single DNA strands are electrophoretically translocated through a nanopore and the resulting ionic signal is used to identify the four DNA bases, an enzyme has been used to ratchet the nucleic acid stepwise through the pore at a controlled speed. In this work, we investigated the ability of alpha-hemolysin nanopores to distinguish the four DNA bases under conditions that are compatible with the activity of DNA-handling enzymes. Our findings suggest that in immobilized strands, the applied potential exerts a force on DNA (∼10 pN at +160 mV) that increases the distance between nucleobases by about 2.2 Å V(-1). The four nucleobases can be resolved over wide ranges of applied potentials (from +60 to +220 mV in 1 m KCl) and ionic strengths (from 200 mM KCl to 1 M KCl at +160 mV) and nucleobase recognition can be improved when the ionic strength on the side of the DNA-handling enzyme is low, while the ionic strength on the opposite side is high.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.