ArticleProceedings of the National Academy of Sciences of the United States of America2015
Haploinsufficiency screen highlights two distinct groups of ribosomal protein genes essential for embryonic stem cell fate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Mechanisms coordinating exit from the stem cell state in mammals.Genes & development · 2026Review
- Emerging roles of ribosome translation in stem cells and stem cell therapy - a review.Cell & bioscience · 2025Review
- DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.Nature communications · 2024Article
- The ubiquitin-proteasome system regulates the formation of specialized ribosomes during high salt stress in yeast.bioRxiv : the preprint server for biology · 2024Article
- A unique poly(A) tail profile uncovers the stability and translational activation of TOP transcripts during neuronal differentiation.iScience · 2023Article
- Article
- Article
- The chaperone Tsr2 regulates Rps26 release and reincorporation from mature ribosomes to enable a reversible, ribosome-mediated response to stress.Science advances · 2022Article
- Discriminative feature of cells characterizes cell populations of interest by a small subset of genes.PLoS computational biology · 2021Article
- Translational control of stem cell function.Nature reviews. Molecular cell biology · 2021Review
- Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions.Nature methods · 2021Article
- Biological Mechanisms Induced by Soybean Agglutinin Using an Intestinal Cell Model of Monogastric Animals.Frontiers in veterinary science · 2021Article
- Review
- Ribosome Stoichiometry: From Form to Function.Trends in biochemical sciences · 2019Review
- Mouse adult hematopoietic stem cells actively synthesize ribosomal RNA.RNA (New York, N.Y.) · 2018Article
- Key genes and integrated modules in hematopoietic differentiation of human embryonic stem cells: a comprehensive bioinformatic analysis.Stem cell research & therapy · 2018Article
- The ribosome, (slow) beating heart of cancer (stem) cell.Oncogenesis · 2018Review
- Inhibition of post-transcriptional steps in ribosome biogenesis confers cytoprotection against chemotherapeutic agents in a p53-dependent manner.Scientific reports · 2017Article
- A partial loss-of-function mutation in an Arabidopsis RNA polymerase III subunit leads to pleiotropic defects.Journal of experimental botany · 2016Article
- Differential Stoichiometry among Core Ribosomal Proteins.Cell reports · 2015Article
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5 authors.
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Abstract
In a functional genomics screen of mouse embryonic stem cells (ESCs) with nested hemizygous chromosomal deletions, we reveal that ribosomal protein (RP) genes are the most significant haploinsufficient determinants for embryoid body (EB) formation. Hemizygocity for three RP genes (Rps5, Rps14, or Rps28), distinguished by the proximity of their corresponding protein to the ribosome's mRNA exit site, is associated with the most profound phenotype. This EB phenotype was fully rescued by BAC or cDNA complementation but not by the reduction of p53 levels, although such reduction was effective with most other RP-deleted clones corresponding to non-mRNA exit-site proteins. RNA-sequencing studies further revealed that undifferentiated ESCs hemizygous for Rps5 showed reduced expression levels of several mesoderm-specific genes as compared with wild-type counterparts. Together, these results reveal that RP gene dosage limits the differentiation, not the self-renewal, of mouse ESCs. They also highlight two separate mechanisms underlying this process, one of which is p53 independent.
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