ArticleProceedings of the National Academy of Sciences of the United States of America2015
Single liposome analysis of peptide translocation by the ABC transporter TAPL.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Single-molecule dynamics reveal ATP binding alone powers substrate translocation by an ABC transporter.Nature communications · 2026Article
- Interdomain communication in a homodimeric ABC transporter.The Journal of biological chemistry · 2024Article
- Emerging Role of ABC Transporters in Glia Cells in Health and Diseases of the Central Nervous System.Cells · 2024Review
- Biofilm formation: mechanistic insights and therapeutic targets.Molecular biomedicine · 2023Review
- The lysosomal transporter TAPL has a dual role as peptide translocator and phosphatidylserine floppase.Nature communications · 2022Article
- Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins.Membranes · 2021Review
- Mutagenic Analysis of the Putative ABCC6 Substrate-Binding Cavity Using a New Homology Model.International journal of molecular sciences · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
- A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter.eLife · 2020Article
- Peptide translocation by the lysosomal ABC transporter TAPL is regulated by coupling efficiency and activation energy.Scientific reports · 2019Article
- Lysosomal targeting of the ABC transporter TAPL is determined by membrane-localized charged residues.The Journal of biological chemistry · 2019Article
- Review
- Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL.Scientific reports · 2018Article
- Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Moving the Cellular Peptidome by Transporters.Frontiers in cell and developmental biology · 2018Review
- Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structure and Dynamics of Antigenic Peptides in Complex with TAP.Frontiers in immunology · 2017Review
- Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution.Journal of visualized experiments : JoVE · 2016Article
- Mechanism Matters: A Taxonomy of Cell Penetrating Peptides.Trends in biochemical sciences · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP-binding cassette (ABC) transporters use ATP to drive solute transport across biological membranes. Members of this superfamily have crucial roles in cell physiology, and some of the transporters are linked to severe diseases. However, understanding of the transport mechanism, especially of human ABC exporters, is scarce. We reconstituted the human lysosomal polypeptide ABC transporter TAPL, expressed in Pichia pastoris, into lipid vesicles (liposomes) and performed explicit transport measurements. We analyzed solute transport at the single liposome level by monitoring the coincident fluorescence of solutes and proteoliposomes in the focal volume of a confocal microscope. We determined a turnover number of eight peptides per minute, which is two orders of magnitude higher than previously estimated from macroscopic measurements. Moreover, we show that TAPL translocates peptides against a large concentration gradient. Maximal filling is not limited by an electrochemical gradient but by trans-inhibition. Countertransport and reversibility studies demonstrate that peptide translocation is a strictly unidirectional process. Altogether, these data are included in a refined model of solute transport by ABC exporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.