ArticleProceedings of the National Academy of Sciences of the United States of America2015
Tonoplast CBL-CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed, 262 citations in OpenAlex.
- The saltmarsh cordgrass Spartina anglica has novel genomic features that contribute to abiotic stress tolerance.Journal of experimental botany · 2026Article
- Review
- PuCBL9-PuCIPK11 Modulates Stomatal Microfilament Reorganisation in a Phosphorylation-Dependent Manner to Enhance Drought Tolerance in Poplar.Plant, cell & environment · 2026Article
- Potassium Deficiency and Hormone Signalling in Plants.Plant, cell & environment · 2026Review
- Magnesium: An overlooked signalling ion in plant physiology and circadian regulation.Quantitative plant biology · 2026Review
- Calcium signaling-mediated phosphorylation controls zinc allocation in Arabidopsis.Stress biology · 2025Article
- The CtrCBL1/CtrCIPK6 Complex of Citrus Phosphorylates CtrBBX32 to Regulate CtrSTP1-Mediated Sugar Accumulation and Cold Tolerance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- OsCAX1a-Dependent Ca-Mg Balance Is Required for Optimal Growth in Rice.Rice (New York, N.Y.) · 2025Article
- Multiple independent MGR5 alleles contribute to a clinal pattern in leaf magnesium across the distribution of Arabidopsis thaliana.The New phytologist · 2025Article
- Combining ability and heterosis analysis for mineral content in the leafy vegetable Gynandropsis gynandra (L.) Briq.PloS one · 2025Article
- The CaGenes · 2024Article
- The role of CBL-CIPK signaling in plant responses to biotic and abiotic stresses.Plant molecular biology · 2024Review
- Article
- Understanding the salt overly sensitive pathway in Prunus: Identification and characterization of NHX, CIPK, and CBL genes.The plant genome · 2024Article
- Potato calcium sensor modules StCBL3-StCIPK7 and StCBL3-StCIPK24 negatively regulate plant immunity.BMC plant biology · 2024Article
- TORC pathway intersects with a calcium sensor kinase network to regulate potassium sensing inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Calcium signaling in plant mineral nutrition: From uptake to transport.Plant communications · 2023Review
- The CALCINEURIN B-LIKE 4/CBL-INTERACTING PROTEIN 3 module degrades repressor JAZ5 during rose petal senescence.Plant physiology · 2023Article
- Green Synthesis of Iron Oxide (Hematite) Nanoparticles and Their Influence onPlants (Basel, Switzerland) · 2023Article
- Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.Plant physiology · 2023Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although Mg(2+) is essential for a myriad of cellular processes, high levels of Mg(2+) in the environment, such as those found in serpentine soils, become toxic to plants. In this study, we identified two calcineurin B-like (CBL) proteins, CBL2 and CBL3, as key regulators for plant growth under high-Mg conditions. The Arabidopsis mutant lacking both CBL2 and CBL3 displayed severe growth retardation in the presence of excess Mg(2+), implying elevated Mg(2+) toxicity in these plants. Unexpectedly, the cbl2 cbl3 mutant plants retained lower Mg content than wild-type plants under either normal or high-Mg conditions, suggesting that CBL2 and CBL3 may be required for vacuolar Mg(2+) sequestration. Indeed, patch-clamp analysis showed that the cbl2 cbl3 mutant exhibited reduced Mg(2+) influx into the vacuole. We further identified four CBL-interacting protein kinases (CIPKs), CIPK3, -9, -23, and -26, as functionally overlapping components downstream of CBL2/3 in the signaling pathway that facilitates Mg(2+) homeostasis. The cipk3 cipk9 cipk23 cipk26 quadruple mutant, like the cbl2 cbl3 double mutant, was hypersensitive to high-Mg conditions; furthermore, CIPK3/9/23/26 physically interacted with CBL2/3 at the vacuolar membrane. Our results thus provide evidence that CBL2/3 and CIPK3/9/23/26 constitute a multivalent interacting network that regulates the vacuolar sequestration of Mg(2+), thereby protecting plants from Mg(2+) toxicity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.