ArticleFrontiers in microbiology2014
CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Emerging and Underdetected Colonization Factors in EnterotoxigenicMicroorganisms · 2026Review
- One Health Perspective on the EnterotoxigenicMicroorganisms · 2026Review
- Prevalence of noncanonical virulence factors in the adherence and invasion by enterotoxigenicFrontiers in cellular and infection microbiology · 2026Article
- Article
- Genome Sequencing and Assembly of EnterotoxigenicMicroorganisms · 2025Article
- Age-dependent pathogenic profiles of enterotoxigenicFrontiers in public health · 2024Article
- RecombinantPathogens (Basel, Switzerland) · 2023Article
- The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model.PLoS neglected tropical diseases · 2022Article
- Winning the War against Multi-Drug Resistant Diarrhoeagenic Bacteria.Microorganisms · 2019Article
- Murine immunization with CS21 pili or LngA major subunit of enterotoxigenic Escherichia coli (ETEC) elicits systemic and mucosal immune responses and inhibits ETEC gut colonization.Veterinary microbiology · 2017Article
- Genome Sequence of EnterotoxigenicGenome announcements · 2017Article
- Colonization Factors in Enterotoxigenic Escherichia coli Strains in Travelers to Mexico, Guatemala, and India Compared with Children in Houston, Texas.The American journal of tropical medicine and hygiene · 2017Article
- Tracking Bioluminescent ETEC duringFrontiers in cellular and infection microbiology · 2017Article
- Transcriptomic Analysis of the Host Response and Innate Resilience to Enterotoxigenic Escherichia coli Infection in Humans.The Journal of infectious diseases · 2016Article
- Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundEnterotoxigenic Escherichia coli (ETEC) colonize the human intestinal mucosa using pili and non-pili colonization factors (CFs). CS21 (also designated Longus) is one of the most prevalent CFs encoded by a 14 kb lng DNA cluster located in a virulence plasmid of ETEC; yet limited information is available on the prevalence of CS21 positive ETEC isolates in different countries. The aim of this study was to evaluate the prevalence of CS21 among ETEC clinical isolates from Mexican and Bangladeshi children under 5 years old with diarrhea and to determine the phenotypic and genotypic features of these isolates.
methodsETEC clinical isolates positive to lngA gene were characterized by genotype, multidrug-resistance, self-aggregation, biofilm formation, and adherence to HT-29 cell line.
resultsA collection of 303 E. coli clinical isolates were analyzed, the 81.51% (247/303) were identified as ETEC, 30.76% (76/247) were st (+)/lt (+), and 25.10% (62/247) were positive for the lngA gene. Among the lngA (+) ETECs identified, 50% of isolates (31/62) were positive for LngA protein. The most frequent serotype was O128ac:H12 found in 19.35% (12/62) of lngA (+) ETEC studied. Multidrug-resistance (MDR) lngA (+) ETEC isolates was identified in 65% (39/60), self-aggregation in 48.38% (30/62), and biofilm formation in 83.87% (52/62). ETEC lngA (+) isolates were able to adhere to HT-29 cells at different levels. Two lngA isogenic mutants were constructed in the ETEC E9034A and ETEC73332 clinical isolate, showing a 77% and 98% reduction in adherence, respectively with respect to the wild type.
conclusionETEC isolates that have the lngA gene showed features associated with self-aggregation, and adherence to HT-29 cells, important characteristics in the human gut colonization process and pathogenesis.
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