ArticleJournal of nanobiotechnology2015
Investigating the immunomodulatory nature of zinc oxide nanoparticles at sub-cytotoxic levels in vitro and after intranasal instillation in vivo.
Article in Journal of nanobiotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Biosynthesis and Characterization of Zinc Oxide-Doped Selenium Nanocomposite Conjugated With l-Asparaginase Against Human Hepatoma Cell Line.Biotechnology and applied biochemistry · 2026Article
- Broccoli triggers the hemocompatibility of green nanoparticles.Discover nano · 2026Article
- Ginger phytochemical corona enhances hemocompatibility of metal oxide nanoparticles for blood-contacting applications.Scientific reports · 2026Article
- Cutting-edge nanotechnology: unveiling the role of zinc oxide nanoparticles in combating deadly gastrointestinal tumors.Frontiers in bioengineering and biotechnology · 2025Review
- Repeated intratracheal instillation effects of commonly used vehicles in toxicity studies with mice.Scientific reports · 2024Article
- Cytotoxic Potencies of Zinc Oxide Nanoforms in A549 and J774 Cells.Nanomaterials (Basel, Switzerland) · 2024Article
- Differentially Induced Autophagy by Engineered Nanomaterial Treatment Has an Impact on Cellular Homeostasis and Cytotoxicity.Nano letters · 2024Article
- Review of Zinc Oxide Nanoparticles: Toxicokinetics, Tissue Distribution for Various Exposure Routes, Toxicological Effects, Toxicity Mechanism in Mammals, and an Approach for Toxicity Reduction.Biological trace element research · 2024Review
- Cerium Oxide Enhances the Toxicity of Zinc Oxide Nanoparticles in Human Lung Epithelial Cell Cultures.Toxics · 2022Article
- Zinc associated nanomaterials and their intervention in emerging respiratory viruses: Journey to the field of biomedicine and biomaterials.Coordination chemistry reviews · 2022Review
- Zinc intoxication induces ferroptosis in A549 human lung cells.Metallomics : integrated biometal science · 2019Article
- Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Apoptosis and oxidative stress as relevant mechanisms of antitumor activity and genotoxicity of ZnO-NPs alone and in combination with N-acetyl cysteine in tumor-bearing mice.International journal of nanomedicine · 2019Article
- Serum zinc level is associated with liver dysfunction caused by white smoke inhalation.Gastroenterology report · 2018Article
- Neuroinflammation is induced by tongue-instilled ZnO nanoparticles via the CaParticle and fibre toxicology · 2018Article
- An Integrated, High-Throughput Strategy for Multiomic Systems Level Analysis.Journal of proteome research · 2018Article
- A Comparative In Vivo Scrutiny of Biosynthesized Copper and Zinc Oxide Nanoparticles by Intraperitoneal and Intravenous Administration Routes in Rats.Nanoscale research letters · 2018Article
- Global gene expression analysis of macrophage response induced by nonporous and porous silica nanoparticles.Nanomedicine : nanotechnology, biology, and medicine · 2018Article
- Prognostic value of serum zinc levels in patients with acute HC/zinc chloride smoke inhalation.Medicine · 2017Article
- Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner.International journal of nanomedicine · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study evaluates the time-dependent pro-inflammatory response of the model human lung epithelial cells (A549) to industrially relevant zinc oxide nanoparticles (ZnO NPs). In terms of toxicity, ZnO-NPs are categorised into the group of high toxicity nanomaterials. However information on pro-inflammatory potential of these NPs at sub-toxic concentrations is limited. Understanding how cellular defense mechanisms function in the presence of sub-cytotoxic concentrations of these NPs is vital. Moreover, there is an urgent need for additional in vivo studies addressing pulmonary toxicity due to accidental inhalation of ZnO NPs.
resultsExposure to sub-cytotoxic ZnO NP concentrations (20 μg/mL) induced significant up-regulation of mRNA for the pro-inflammatory cytokine IL-8 and redox stress marker heme oxygenase-1, along with increased release of IL-8. The highest pro-inflammatory response was recorded after 4 to 6 hr exposure to ZnO NPs over a 24 hr period. Pre-treatment of A549 cells with the sulfhydryl antioxidant N-acetyl cysteine (at 5 mM) resulted in significant reduction of the up-regulation of inflammatory markers, confirming the role of reactive oxygen species in the observed immunomodulatory effects, independent of cytotoxicity. Furthermore, we report for the first time that, intranasal instillation of a single dose (5 mg/kg) of pristine or surfactant-dispersed ZnO NPs can cause pulmonary inflammation, already after 24 hr in a murine model. This was confirmed by up-regulation of eotaxin mRNA in the lung tissue and release of pro-inflammatory cytokines in the sera of mice exposed to ZnO NPs.
conclusionOur study highlights that even at sub-cytotoxic doses ZnO NPs can stimulate a strong inflammatory and antioxidant response in A549 cells. ZnO NP mediated cytotoxicity may be the outcome of failure of cellular redox machinery to contain excessive ROS formation. Moreover exposure to a single but relatively high dose of ZnO NPs via intranasal instillation may provoke acute pulmonary inflammatory reactions in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.