ArticleAntimicrobial agents and chemotherapy2015
Autophagy regulates colistin-induced apoptosis in PC-12 cells.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Corynoxeine Supplementation Ameliorates Colistin-Induced Kidney Oxidative Stress and Inflammation in Mice.Antioxidants (Basel, Switzerland) · 2025Article
- Examining the role of trans-sodium crocetinate in alleviating colistin-induced cytotoxicity through apoptosis and autophagy pathways on HEK-293 cells.Iranian journal of basic medical sciences · 2025Article
- The power of trans-sodium crocetinate: exploring its renoprotective effects in a rat model of colistin-induced nephrotoxicity.Naunyn-Schmiedeberg's archives of pharmacology · 2024Article
- Colistin Induces Oxidative Stress and Apoptotic Cell Death through the Activation of the AhR/CYP1A1 Pathway in PC12 Cells.Antioxidants (Basel, Switzerland) · 2024Article
- Modification of the Antibiotic, Colistin, with Dextrin Causes Enhanced Cytotoxicity and Triggers Apoptosis in Myeloid Leukemia.International journal of nanomedicine · 2024Article
- Colistin Effects on Emphysematous Lung in an LPS-Sepsis Model.Antibiotics (Basel, Switzerland) · 2023Article
- Critical-Illness: Combined Effects of Colistin and Vasoactive Drugs: A Pilot Study.Antibiotics (Basel, Switzerland) · 2023Article
- Pyroptosis, apoptosis, and autophagy are involved in infection induced by two clinicalFrontiers in cellular and infection microbiology · 2023Article
- IGF-1 Alleviates Mitochondrial Apoptosis through the GSK3β/NF-κB/NLRP3 Signaling Pathway in LPS-Treated PC-12 Cells.Journal of molecular neuroscience : MN · 2021Article
- Polymyxins-Curcumin Combination Antimicrobial Therapy: Safety Implications and Efficacy for Infection Treatment.Antioxidants (Basel, Switzerland) · 2020Review
- Colistin induced peripheral neurotoxicity involves mitochondrial dysfunction and oxidative stress in mice.Molecular biology reports · 2019Article
- Baicalin AttenuatesInfection and drug resistance · 2019Article
- Immunomodulatory Effects of Colistin on Macrophages in Rats by Activating the p38/MAPK Pathway.Frontiers in pharmacology · 2019Article
- Intracellular localization of polymyxins in human alveolar epithelial cells.The Journal of antimicrobial chemotherapy · 2019Article
- Polymyxin B causes DNA damage in HK-2 cells and mice.Archives of toxicology · 2018Article
- Adoptive Autophagy Activation: a Much-Needed Remedy Against Chemical Induced Neurotoxicity/Developmental Neurotoxicity.Molecular neurobiology · 2017Review
- A Dual Role of P53 in Regulating Colistin-Induced Autophagy in PC-12 Cells.Frontiers in pharmacology · 2017Article
- Snail-activated long non-coding RNA PCA3 up-regulates PRKD3 expression by miR-1261 sponging, thereby promotes invasion and migration of prostate cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- p53 Mediates Colistin-Induced Autophagy and Apoptosis in PC-12 Cells.Antimicrobial agents and chemotherapy · 2016Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colistin is a cyclic cationic polypeptide antibiotic with activity against multidrug-resistant Gram-negative bacteria. Our recent study demonstrated that colistin induces apoptosis in primary chick cortex neurons and PC-12 cells. Although apoptosis and autophagy have different impacts on cell fate, there is a complex interaction between them. Autophagy plays an important role as a homeostasis regulator by removing excessive or unnecessary proteins and damaged organelles. The aim of the present study was to investigate the modulation of autophagy and apoptosis regulation in PC-12 cells in response to colistin treatment. PC-12 cells were exposed to colistin (125 to 250 μg/ml), and autophagy was detected by visualization of monodansylcadaverine (MDC)-labeled vacuoles, LC3 (microtubule-associated protein 1 light chain 3) immunofluorescence microscopic examination, and Western blotting. Apoptosis was measured by flow cytometry, Hoechst 33258 staining, and Western blotting. Autophagosomes were observed after treatment with colistin for 12 h, and the levels of LC3-II gene expression were determined; observation and protein levels both indicated that colistin induced a high level of autophagy. Colistin treatment also led to apoptosis in PC-12 cells, and the level of caspase-3 expression increased over the 24-h period. Pretreatment of cells with 3-methyladenine (3-MA) increased colistin toxicity in PC-12 cells remarkably. However, rapamycin treatment significantly increased the expression levels of LC3-II and beclin 1 and decreased the rate of apoptosis of PC-12 cells. Our results demonstrate that colistin induced autophagy and apoptosis in PC-12 cells and that the latter was affected by the regulation of autophagy. It is very likely that autophagy plays a protective role in the reduction of colistin-induced cytotoxicity in neurons.
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