ReviewWiley interdisciplinary reviews. Developmental biology
Strength in numbers: quantitative single-molecule RNA detection assays.
Review in Wiley interdisciplinary reviews. Developmental biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluation of the Suitability of RNAscope as a Technique to Measure Gene Expression in Clinical Diagnostics: A Systematic Review.Molecular diagnosis & therapy · 2022Pooled it
- Spatial tissue analysis of secondary sarcoma following CAR T cell therapy for B cell lymphoma.iScience · 2026Article
- Article
- Multiplex Fluorescent mRNA In Situ Hybridization of Developing Craniofacial Tissues in FFPE Mouse Samples.Journal of visualized experiments : JoVE · 2026Article
- A fastFly · 2025Article
- Dual roles of salivary proteins in feeding and silk fiber coating in the spider mite Tetranychus urticae.Communications biology · 2025Article
- Non-Coding RNA-Mediated Gene Regulation in Cardiovascular Disorders: Current Insights and Future Directions.Journal of cardiovascular translational research · 2024Review
- Absolute Quantification of mRNA Isoforms in Adult Stem Cells Using Microfluidic Digital PCR.Bio-protocol · 2023Article
- New discoveries in the field of metabolism by applying single-cell and spatial omics.Journal of pharmaceutical analysis · 2023Review
- SUFI: an automated approach to spectral unmixing of fluorescent multiplex images captured in mouse and post-mortem human brain tissues.BMC neuroscience · 2023Article
- Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Advances in Aptamers-Based Applications in Breast Cancer: Drug Delivery, Therapeutics, and Diagnostics.International journal of molecular sciences · 2022Review
- Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis.Nature communications · 2022Article
- Recent advances in tools to map the microbiome.Current opinion in biomedical engineering · 2021Article
- Annealed ZnO/AlNanomaterials (Basel, Switzerland) · 2021Article
- smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods.Communications biology · 2021Article
- Demystifying biotrophs: FISHing for mRNAs to decipher plant and algal pathogen-host interaction at the single cell level.Scientific reports · 2020Article
- Single-cell and spatial transcriptomics approaches of cardiovascular development and disease.BMB reports · 2020Review
- Bayesian Estimation for Stochastic Gene Expression Using Multifidelity Models.The journal of physical chemistry. B · 2019Article
- Single-Cell RNA Sequencing of the Cardiovascular System: New Looks for Old Diseases.Frontiers in cardiovascular medicine · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledGene expression is a fundamental process that underlies development, homeostasis, and behavior of organisms. The fact that it relies on nucleic acid intermediates, which can specifically interact with complementary probes, provides an excellent opportunity for studying the multiple steps--transcription, RNA processing, transport, translation, degradation, and so forth--through which gene function manifests. Over the past three decades, the toolbox of nucleic acid science has expanded tremendously, making high-precision in situ detection of DNA and RNA possible. This has revealed that many--probably the vast majority of--transcripts are distributed within the cytoplasm or the nucleus in a nonrandom fashion. With the development of microscopy techniques we have learned not only about the qualitative localization of these molecules but also about their absolute numbers with great precision. Single-molecule techniques for nucleic acid detection have been transforming our views of biology with elementary power: cells are not average members of their population but are highly distinct individuals with greatly and suddenly changing gene expression, and this behavior of theirs can be measured, modeled, and thus predicted and, finally, comprehended. For further resources related to this article, please visit the WIREs website. CONFLICT OF INTEREST: The authors have declared no conflicts of interest for this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.