ArticleJournal of industrial microbiology & biotechnology2015
Synthesis of medium-chain length (C6-C10) fuels and chemicals via β-oxidation reversal in Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Reverse beta-oxidation for biochemical production: insights into the functional properties of key enzymes.BMB reports · 2026Review
- A metabolic engineering strategy for producing poly-(3-hydroxyoctanoic acid) in Escherichia coli from glycerol.Metabolic engineering · 2025Article
- Optimizing hexanoic acid biosynthesis in Saccharomyces cerevisiae for the de novo production of olivetolic acid.Biotechnology for biofuels and bioproducts · 2024Article
- Biosynthesis of C4-C8 3-Hydroxycarboxylic Acids from Glucose through the Inverted Fatty Acid β-Oxidation by Metabolically EngineeredBiomolecules · 2024Article
- Relative Activities of the β-ketoacyl-CoA and Acyl-CoA Reductases Influence Product Profile and Flux in a Reversed β-Oxidation Pathway.ACS catalysis · 2023Article
- An optimized reverse β-oxidation pathway to produce selected medium-chain fatty acids in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli.Biotechnology and bioengineering · 2022Article
- Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria.Nature communications · 2022Article
- Reverse β-oxidation pathways for efficient chemical production.Journal of industrial microbiology & biotechnology · 2022Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds.Applied microbiology and biotechnology · 2020Review
- Production of 1-octanol in Escherichia coli by a high flux thioesterase route.Metabolic engineering · 2020Article
- Increased Accumulation of Medium-Chain Fatty Acids by Dynamic Degradation of Long-Chain Fatty Acids inGenes · 2020Article
- Synthetic Biology and Metabolic Engineering EmployingFrontiers in bioengineering and biotechnology · 2020Review
- A Review of the Biotechnological Production of Methacrylic Acid.Frontiers in bioengineering and biotechnology · 2020Review
- The isoprenoid alcohol pathway, a synthetic route for isoprenoid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) inInternational journal of molecular sciences · 2019Article
- Highly Active CACS synthetic biology · 2018Article
- Advances in bio-based production of dicarboxylic acids longer than C4.Engineering in life sciences · 2018Review
- Rational design of thiolase substrate specificity for metabolic engineering applications.Biotechnology and bioengineering · 2018Article
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3 authors.
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Abstract
The recently engineered reversal of the β-oxidation cycle has been proposed as a potential platform for the efficient synthesis of longer chain (C ≥ 4) fuels and chemicals. Here, we demonstrate the utility of this platform for the synthesis of medium-chain length (C6-C10) products through the manipulation of key components of the pathway. Deletion of endogenous thioesterases provided a clean background in which the expression of various thiolase and termination components, along with required core enzymes, resulted in the ability to alter the chain length distribution and functionality of target products. This approach enabled the synthesis of medium-chain length carboxylic acids and primary alcohols from glycerol, a low-value feedstock. The use of BktB as the thiolase component with thioesterase TesA' as the termination enzyme enabled the synthesis of about 1.3 g/L C6-C10 saturated carboxylic acids. Tailoring of product formation to primary alcohol synthesis was achieved with the use of various acyl-CoA reductases. The combination of AtoB and FadA as the thiolase components with the alcohol-forming acyl-CoA reductase Maqu2507 from M. aquaeolei resulted in the synthesis of nearly 0.3 g/L C6-C10 alcohols. These results further demonstrate the versatile nature of a β-oxidation reversal, and highlight several key aspects and control points that can be further manipulated to fine-tune the synthesis of various fuels and chemicals.
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