ArticleJournal of industrial microbiology & biotechnology2015
Quantifying the sensitivity of G. oxydans ATCC 621H and DSM 3504 to osmotic stress triggered by soluble buffers.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy.Journal of biological engineering · 2025Article
- Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates.BMC biotechnology · 2023Article
- Highly efficient fermentation of 5-keto-D-fructose with Gluconobacter oxydans at different scales.Microbial cell factories · 2022Article
- Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands.Journal of biological engineering · 2022Article
- Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria.Biosensors · 2021Article
- Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose.Microbial cell factories · 2020Article
- Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask.Microbial cell factories · 2018Article
- Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.Journal of biological engineering · 2015Article
- Design and Characterization of an Osmotic Pump System for Optimal Feeding and pH Control inIranian journal of pharmaceutical research : IJPRArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Gluconobacter oxydans cultivations on glucose, CaCO3 is typically used as pH-buffer. This buffer, however, has disadvantages: suspended CaCO3 particles make the medium turbid, thereby, obstructing analysis of microbial growth via optical density and scattered light. Upon searching for alternative soluble pH-buffers, bacterial growth and productivity was inhibited most probably due to osmotic stress. Thus, this study investigates in detail the osmotic sensitivity of G. oxydans ATCC 621H and DSM 3504 using the Respiratory Activity MOnitoring System. The tested soluble pH-buffers and other salts attained osmolalities of 0.32-1.19 osmol kg(-1). This study shows that G. oxydans ATCC 621H and DSM 3504 respond quite sensitively to increased osmolality in comparison to other microbial strains of industrial interest. Osmolality values of >0.5 osmol kg(-1) should not be exceeded to avoid inhibition of growth and product formation. This osmolality threshold needs to be considered when working with soluble pH-buffers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.