ArticleBiochemical and biophysical research communications2015
Ascorbic acid transport in brain microvascular pericytes.
Article in Biochemical and biophysical research communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Administration of Ascorbic Acid Alleviates Neuronal Damage After Cerebral Ischemia in ODS Rats.Antioxidants (Basel, Switzerland) · 2025Article
- Vitamin C alleviates hyperglycemic stress in retinal pigment epithelial cells.Molecular biology reports · 2024Article
- Oxidative Stress in Traumatic Brain Injury.International journal of molecular sciences · 2022Review
- Ascorbate Inhibits Proliferation and Promotes Myeloid Differentiation inFrontiers in oncology · 2021Article
- Vitamin C Transporters and Their Implications in Carcinogenesis.Nutrients · 2020Review
- Antioxidant Therapies in Traumatic Brain Injury.Antioxidants (Basel, Switzerland) · 2020Review
- Water- and Fat-Soluble Antioxidants in Human Seminal Plasma and Serum of Fertile Males.Antioxidants (Basel, Switzerland) · 2019Article
- SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke.Frontiers in cellular neuroscience · 2019Article
- Review
- Ascorbic acid repletion: A possible therapy for diabetic macular edema?Free radical biology & medicine · 2016Review
- Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin.The Journal of biological chemistry · 2015Article
- Ascorbic acid efflux from human brain microvascular pericytes: role of re-uptake.BioFactors (Oxford, England)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular vitamin C, or ascorbic acid, has been shown to prevent the apoptosis of cultured vascular pericytes under simulated diabetic conditions. We sought to determine the mechanism by which ascorbate is transported into pericytes prior to exerting this protective effect. Measuring intracellular ascorbate, we found that pericytes display a linear uptake over 30 min and an apparent transport Km of 21 μM, both of which are consistent with activity of the Sodium-dependent Vitamin C Transporter 2 (SVCT2). Uptake of both radiolabeled and unlabeled ascorbate was prevented by inhibiting SVCT2 activity, but not by inhibiting the activity of GLUT-type glucose transporters, which import dehydroascorbate to also generate intracellular ascorbate. Likewise, uptake of dehydroascorbate was prevented with the inhibition of GLUTs, but not by inhibiting the SVCT2, indicating substrate specificity of both transporters. Finally, presence of the SVCT2 in pericytes was confirmed by western blot analysis, and immunocytochemistry was used to localize it to the plasma membrane and intracellular sites. Together, these data clarify previous inconsistencies in the literature, implicate SVCT2 as the pericyte ascorbate transporter, and show that pericytes are capable of concentrating intracellular ascorbate against a gradient in an energy- and sodium-dependent fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.