ArticleGenes & development2015
Structure of the apoptosome: mechanistic insights into activation of an initiator caspase from Drosophila.
Article in Genes & development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- Filamentation activates bacterial Avs5 antiviral protein.Nature communications · 2025Article
- Dark and Dronc activation inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural insights into CED-3 activation.Life science alliance · 2023Article
- Wg/Wnt1 and Erasp link ER stress to proapoptotic signaling in an autosomal dominant retinitis pigmentosa model.Experimental & molecular medicine · 2023Article
- Mechanism of NAIP-NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5.Nature structural & molecular biology · 2023Article
- Prokaryotic innate immunity through pattern recognition of conserved viral proteins.Science (New York, N.Y.) · 2022Article
- Structure of an inactive RNA polymerase II dimer.Nucleic acids research · 2021Article
- Mycobacterial STAND adenylyl cyclases: The HTH domain binds DNA to form biocrystallized nucleoids.Biophysical journal · 2021Article
- Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine.Scientific reports · 2021Article
- Identification and Functional Analysis of Apoptotic Protease Activating Factor-1 (Apaf-1) fromInsects · 2021Article
- Structure of the activated ROQ1 resistosome directly recognizing the pathogen effector XopQ.Science (New York, N.Y.) · 2020Article
- Accurate elimination of superfluous attachment cells is critical for the construction of functional multicellular proprioceptors in Drosophila.Cell death and differentiation · 2019Article
- Necrosis, apoptosis, necroptosis, three modes of action of dopaminergic neuron neurotoxins.PloS one · 2019Article
- NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.Nature communications · 2018Article
- Involvement of NF-κB in mediating the anti-tumour effects of combretastatins in T cells.Investigational new drugs · 2018Article
- New insights into apoptosome structure and function.Cell death and differentiation · 2018Review
- Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.Cell death and differentiation · 2018Review
- Article
- Proapoptotic function of deubiquitinaseOncotarget · 2017Article
- p53-dependent programmed necrosis controls germ cell homeostasis during spermatogenesis.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Apoptosis is executed by a cascade of caspase activation. The autocatalytic activation of an initiator caspase, exemplified by caspase-9 in mammals or its ortholog, Dronc, in fruit flies, is facilitated by a multimeric adaptor complex known as the apoptosome. The underlying mechanism by which caspase-9 or Dronc is activated by the apoptosome remains unknown. Here we report the electron cryomicroscopic (cryo-EM) structure of the intact apoptosome from Drosophila melanogaster at 4.0 Å resolution. Analysis of the Drosophila apoptosome, which comprises 16 molecules of the Dark protein (Apaf-1 ortholog), reveals molecular determinants that support the assembly of the 2.5-MDa complex. In the absence of dATP or ATP, Dronc zymogen potently induces formation of the Dark apoptosome, within which Dronc is efficiently activated. At 4.1 Å resolution, the cryo-EM structure of the Dark apoptosome bound to the caspase recruitment domain (CARD) of Dronc (Dronc-CARD) reveals two stacked rings of Dronc-CARD that are sandwiched between two octameric rings of the Dark protein. The specific interactions between Dronc-CARD and both the CARD and the WD40 repeats of a nearby Dark protomer are indispensable for Dronc activation. These findings reveal important mechanistic insights into the activation of initiator caspase by the apoptosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.