ArticleExtremophiles : life under extreme conditions2015
Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus.
Article in Extremophiles : life under extreme conditions, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- Intermolecular Gene Conversion for the Equalization of Genome Copies in the Polyploid HaloarchaeonGenes · 2024Article
- Article
- DNA repair enzymes of the Antarctic Dry Valley metagenome.Frontiers in microbiology · 2023Article
- Genetic Study of Four Candidate Holliday Junction Processing Proteins in the Thermophilic CrenarchaeonInternational journal of molecular sciences · 2022Article
- Review
- Functional Analysis of the NucS/EndoMS of the Hyperthermophilic ArchaeonFrontiers in microbiology · 2020Article
- Phosphorylation of the Archaeal Holliday Junction Resolvase Hjc Inhibits Its Catalytic Activity and Facilitates DNA Repair inFrontiers in microbiology · 2019Article
- The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction.Nucleic acids research · 2018Article
- Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration.Journal of molecular biology · 2017Article
- Hydroxyurea-Mediated Cytotoxicity Without Inhibition of Ribonucleotide Reductase.Cell reports · 2016Article
- Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A.Extremophiles : life under extreme conditions · 2016Article
- Understanding DNA Repair in Hyperthermophilic Archaea: Persistent Gaps and Other Reasons to Focus on the Fork.Archaea (Vancouver, B.C.) · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The in vivo functions of Hje and Hjc, two Holliday junction resolvases in Sulfolobus islandicus were investigated. We found that deletion of either hje or hjc had no effect on normal cell growth, while deletion of both hje and hjc is lethal. Although Hjc is the conserved resolvase in all archaea, the hje deletion rather than hjc deletion rendered cells more sensitive to DNA-damaging agents such as hydroxyurea, cisplatin, and methyl methanesulfonate than the wild type (WT). Intriguingly, the sensitivity of Δhje could not be rescued by ectopic expression of Hje from a plasmid and Hje overexpression slowed growth and large cells appeared with more than two genome equivalents. We showed that Hje was maintained at a low level in WT cells. Furthermore, transcriptomic microarray analysis revealed that the abundance of transcripts of many genes including those involved in DNA replication, repair, transcription regulation, and cell division changed drastically in the Hje-overexpressed strain. However, only limited genes were up- or downregulated in the hje deletion strain. Our findings collectively suggest that Hje is the primary resolvase involved in DNA repair and its expression must be tightly controlled in cells.
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