ArticleMicrobial biotechnology2015
Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains.
Article in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 90 citations in OpenAlex.
- Identification of MscS as a Key L-Glutamate Exporter in Bacillus methanolicus.Microbial biotechnology · 2025Article
- CRISPR-Cas9-driven genome editing inFrontiers in microbiology · 2025Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol.Microbial biotechnology · 2023Article
- Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production.Microbial biotechnology · 2022Review
- Improved succinic acid production through the reconstruction of methanol dissimilation in Escherichia coli.Bioresources and bioprocessing · 2022Article
- Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients.Frontiers in bioengineering and biotechnology · 2022Review
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Translating advances in microbial bioproduction to sustainable biotechnology.Frontiers in bioengineering and biotechnology · 2022Review
- Ameliorating end-product inhibition to improve cadaverine production in engineeredSynthetic and systems biotechnology · 2021Article
- Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine.Engineering in life sciences · 2021Article
- Green chemical and biological synthesis of cadaverine: recent development and challenges.RSC advances · 2021Review
- Developing a Riboswitch-Mediated Regulatory System for Metabolic Flux Control in ThermophilicInternational journal of molecular sciences · 2021Article
- Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial WorkhorseFrontiers in microbiology · 2021Article
- Genomic and Transcriptomic Investigation of the Physiological Response of the MethylotrophFrontiers in microbiology · 2021Article
- Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases ofFrontiers in microbiology · 2021Article
- Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by ThermophilicFrontiers in bioengineering and biotechnology · 2021Article
- Identification of a cytosine methyltransferase that improves transformation efficiency in Methylomonas sp. DH-1.Biotechnology for biofuels · 2020Article
- Diamine Biosynthesis: Research Progress and Application Prospects.Applied and environmental microbiology · 2020Review
- Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus.mSystems · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l(-1) during shake flask conditions. A volume-corrected concentration of 11.3 g l(-1) of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.