Evidence map›Paper›PMID 25643733›Full record

ArticleVeterinary and comparative oncology2016

Osteosarcoma tissues and cell lines from patients with differing serum alkaline phosphatase concentrations display minimal differences in gene expression patterns.

L C de Sá Rodrigues, K E Holmes, V Thompson, C M Piskun, S E Lana, M A Newton, T J Stein

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary and comparative oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

L C de Sá RodriguesDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
K E HolmesDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
V ThompsonDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
C M PiskunDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
S E LanaFlint Animal Cancer Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft Collins, CO, USA.
M A NewtonDepartments of Statistics and of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI, USA.
T J SteinDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
University of Wisconsin–Madison · USColorado State University · USUniversity of Wisconsin Carbone Cancer Center · US

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI AMY TRENTHAM-DIETZ · 1985 to 2026
$142.6M
Institutional Clinical and Translational Science AwardUL1TR000427 · NCATS · UNIVERSITY OF WISCONSIN-MADISON · PI DREZNER, MARC KENNETH · 2012 to 2016
$32.2M
INSTITUTIONAL CTSA (UW-MADISON): CLINICAL TRIALSUL1RR025011 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI DREZNER, MARC KENNETH · 2007 to 2011
$29.5M
Short-Term Research Training of Veterinary Students in WisconsinT35OD011078 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI BJORLING, DALE EDMOND, JORGENSEN, JOAN S · 2012 to 2025
$1.0M
RESEARCH TRAINING OF VETERINARY STUDENTS IN WISCONSINT32RR017503 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI BJORLING, DALE EDMOND · 2003 to 2011
$774k
NCATS NIH HHS UL1 TR000427NCI NIH HHS P30 CA014520NCRR NIH HHS T32 RR017503NCRR NIH HHS UL1 RR025011NIH HHS T35 OD011078
6 · The paper itself

Abstract

Serum alkaline phosphatase (ALP) concentration is a prognostic factor for osteosarcoma in multiple studies, although its biological significance remains incompletely understood. To determine whether gene expression patterns differed in osteosarcoma from patients with differing serum ALP concentrations, microarray analysis was performed on 18 primary osteosarcoma samples and six osteosarcoma cell lines from dogs with normal and increased serum ALP concentration. No differences in gene expression patterns were noted between tumours or cell lines with differing serum ALP concentration using a gene-specific two-sample t-test. Using a more sensitive empirical Bayes procedure, defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was increased in both the tissue and cell lines of the normal ALP group. Using quantitative PCR (qPCR), differences in DCUN1D1 expression between the two groups failed to reach significance. The homogeneity of gene expression patterns of osteosarcoma associated differing serum ALP concentrations are consistent with previous studies suggesting serum ALP concentration is not associated with intrinsic differences of osteosarcoma cells.

Indexed as

Alkaline PhosphataseAmputation, SurgicalAnimalsAntineoplastic AgentsCell Line, TumorDog DiseasesDogsFemaleGene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticMaleOsteosarcomaAlkaline PhosphataseAntineoplastic Agentscell signallingcomparative oncologygeneticsoncologytumour biology

Identifiers

PMID25643733
PMCPMC4841739
OpenAlexW2123371809

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.