ArticleNature chemical biology2015
Creating small transcription activating RNAs.
Article in Nature chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed.
- Crowdsourced riboregulators reveal design principles for programmable RNA switching.bioRxiv : the preprint server for biology · 2026Article
- RATEX: A Scalable RNA-Based Platform for Logical and Multi-Layered Cellular Programming.Angewandte Chemie (International ed. in English) · 2026Article
- The interaction between the RNA aptamer Dap1 and DasR mirrors DNA operator site recognition in GntR repressors.Nucleic acids research · 2026Article
- Burden-aware feedback control of microbial consortia.Nature communications · 2026Article
- RNA-Based Communication in Heterogeneous Populations of Cell Mimics.ACS synthetic biology · 2026Article
- Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactions.Nucleic acids research · 2026Article
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Designing molecular RNA switches with Restricted Boltzmann machines.Nature communications · 2025Article
- Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.Nature chemistry · 2025Article
- Programmable trans-splicing riboregulators for complex cellular logic computation.Nature chemical biology · 2025Article
- Article
- Feed-forward loop improves the transient dynamics of an antithetic biological controller.Journal of the Royal Society, Interface · 2025Article
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems.Journal of biological engineering · 2024Article
- Ten Years of the Synthetic Biology Summer Course at Cold Spring Harbor Laboratory.ACS synthetic biology · 2024Review
- Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs.Nucleic acids research · 2024Article
- PACRAT: pathogen detection with aptamer-observed cascaded recombinase polymerase amplification-in vitro transcription.RNA (New York, N.Y.) · 2024Article
- Regulatory RNAs inSynthetic and systems biotechnology · 2024Review
- Enzymatic Assembly of Small Synthetic Genes with Repetitive Elements.ACS synthetic biology · 2024Article
- RNA-based controllers for engineering gene and cell therapies.Current opinion in biotechnology · 2024Review
44 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We expanded the mechanistic capability of small RNAs by creating an entirely synthetic mode of regulation: small transcription activating RNAs (STARs). Using two strategies, we engineered synthetic STAR regulators to disrupt the formation of an intrinsic transcription terminator placed upstream of a gene in Escherichia coli. This resulted in a group of four highly orthogonal STARs that had up to 94-fold activation. By systematically modifying sequence features of this group, we derived design principles for STAR function, which we then used to forward engineer a STAR that targets a terminator found in the Escherichia coli genome. Finally, we showed that STARs could be combined in tandem to create previously unattainable RNA-only transcriptional logic gates. STARs provide a new mechanism of regulation that will expand our ability to use small RNAs to construct synthetic gene networks that precisely control gene expression.
Indexed as
Identifiers
25643173What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.