ArticleNature methods2015
A sensor for quantification of macromolecular crowding in living cells.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed.
- Dynamic hinge-motion of PfRIPR revealed by malaria invasion inhibitory antibodies.Nature communications · 2026Article
- Mimetics of in-cell and subcellular crowding and solvation for protein folding.Biochemical Society transactions · 2026Review
- Chemical interactions in polyethylene glycol-induced condensates lead to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor.bioRxiv : the preprint server for biology · 2026Article
- The endoplasmic reticulum displays high polarity with low protein aggregation in human cells.Communications biology · 2026Article
- Stability of non-canonical nucleic acid structure as a potential modulator of cell fate.Nucleic acids research · 2026Review
- FRET-based biosensor moxCRONOS enables quantitative monitoring of macromolecular crowding in organelles and protein aggregates.Journal of biochemistry · 2025Article
- Biomolecular condensates: molecular structure, biological functions, diseases, and therapeutic targets.Molecular biomedicine · 2025Review
- Article
- A coarse-grained model for disordered proteins under crowded conditions.Protein science : a publication of the Protein Society · 2025Article
- Label-Free Quantification of Protein Density in Living Cells.Current protocols · 2025Article
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Stress sensing and response through biomolecular condensates in plants.Plant communications · 2025Review
- Molecular condensation of the CO/NF-YB/NF-YC/FT complex gates floral transition in Arabidopsis.The EMBO journal · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Article
- Measurement of protein concentration in bacteria and small organelles under a light transmission microscope.Journal of molecular recognition : JMR · 2024Article
- Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level.ACS sensors · 2024Article
- Hypersensitivity of the vimentin cytoskeleton to net-charge states and Coulomb repulsion.bioRxiv : the preprint server for biology · 2024Article
- High-viscosity driven modulation of biomechanical properties of human mesenchymal stem cells promotes osteogenic lineage.Materials today. Bio · 2024Article
- Contribution of different macromolecules to the diffusion of a 40 nm particle in Escherichia coli.Biophysical journal · 2024Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.