ArticlePLoS genetics2015
A nitric oxide regulated small RNA controls expression of genes involved in redox homeostasis in Bacillus subtilis.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Analysis of Bacillus subtilis Ribonuclease Activity In Vivo.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A nitrite-responsive regulatory RNA locus sustains commensal resilience against nitrosative stress.bioRxiv : the preprint server for biology · 2026Article
- Metastable Folding of Bacillus subtilis glmS Ribozyme Modulates Turnover by RNase J1.Journal of molecular biology · 2026Article
- What makes a small RNA work?Nucleic acids research · 2025Review
- A small regulatory RNA controls antibiotic adaptation inAntimicrobial agents and chemotherapy · 2025Article
- A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis.Nucleic acids research · 2025Article
- Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus.PLoS genetics · 2024Article
- Exploring the targetome of IsrR, an iron-regulated sRNA controlling the synthesis of iron-containing proteins inFrontiers in microbiology · 2024Article
- Unveiling the orchestration: mycobacterial small RNAs as key mediators in host-pathogen interactions.Frontiers in microbiology · 2024Review
- Methodologies for bacterial ribonuclease characterization using RNA-seq.FEMS microbiology reviews · 2023Review
- New RoxS sRNA Targets Identified in Bacillus subtilis by Pulsed SILAC.Microbiology spectrum · 2023Article
- Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs.Nature communications · 2023Article
- Thirty Years of sRNA-Mediated Regulation inInternational journal of molecular sciences · 2022Review
- Interrelation between Stress Management and Secretion Systems ofPathogens (Basel, Switzerland) · 2022Article
- RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression.Nature communications · 2022Article
- A new role for SR1 from Bacillus subtilis: regulation of sporulation by inhibition of kinA translation.Nucleic acids research · 2021Article
- Review
- Identification of an RNA sponge that controls the RoxS riboregulator of central metabolism in Bacillus subtilis.Nucleic acids research · 2021Article
- Transcriptional and Post-transcriptional Control of the Nitrate Respiration in Bacteria.Frontiers in molecular biosciences · 2021Review
- Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection.Genes · 2020Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RsaE is the only known trans-acting small regulatory RNA (sRNA) besides the ubiquitous 6S RNA that is conserved between the human pathogen Staphylococcus aureus and the soil-dwelling Firmicute Bacillus subtilis. Although a number of RsaE targets are known in S. aureus, neither the environmental signals that lead to its expression nor its physiological role are known. Here we show that expression of the B. subtilis homolog of RsaE is regulated by the presence of nitric oxide (NO) in the cellular milieu. Control of expression by NO is dependent on the ResDE two-component system in B. subtilis and we determined that the same is true in S. aureus. Transcriptome and proteome analyses revealed that many genes with functions related to oxidative stress and oxidation-reduction reactions were up-regulated in a B. subtilis strain lacking this sRNA. We have thus renamed it RoxS. The prediction of RoxS-dependent mRNA targets also suggested a significant enrichment for mRNAs related to respiration and electron transfer. Among the potential direct mRNA targets, we have validated the ppnKB mRNA, encoding an NAD+/NADH kinase, both in vivo and in vitro. RoxS controls both translation initiation and the stability of this transcript, in the latter case via two independent pathways implicating RNase Y and RNase III. Furthermore, RNase Y intervenes at an additional level by processing the 5' end of the RoxS sRNA removing about 20 nucleotides. Processing of RoxS allows it to interact more efficiently with a second target, the sucCD mRNA, encoding succinyl-CoA synthase, thus expanding the repertoire of targets recognized by this sRNA.
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