Evidence map›Paper›PMID 25642712›Full record

ArticlePloS one2015

Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica.

Keisuke Okada, Hiroyuki Abe, Fumio Ike, Yoshitoshi Ogura, Tetsuya Hayashi, Aya Fukui-Miyazaki, Keiji Nakamura, Naoaki Shinzawa, Yasuhiko Horiguchi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Characterisation ofEpidemiology and infection · 2020
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Keisuke OkadaDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Hiroyuki AbeDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Fumio IkeExperimental Animal Division, RIKEN BioResource Center, Ibaraki, Japan.
Yoshitoshi OguraDivision of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan; Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Tetsuya HayashiDivision of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan; Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Aya Fukui-MiyazakiDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Keiji NakamuraDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Naoaki ShinzawaDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Yasuhiko HoriguchiDepartment of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Osaka University · JPUniversity of Miyazaki · JPRIKEN BioResource Research Center · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bordetella bronchiseptica is a pathogenic bacterium causing respiratory infections in a broad range of mammals. Recently, we determined the whole genome sequence of B. bronchiseptica S798 strain isolated from a pig infected with atrophic rhinitis and found four single-nucleotide polymorphisms (SNPs) at positions -129, -72, +22, and +38 in the region upstream of dnt encoding dermonecrotic toxin (DNT), when compared with a rabbit isolate, RB50. DNT is known to be involved in turbinate atrophy observed in atrophic rhinitis. Immunoblotting, quantitative real-time PCR, and β-galactosidase reporter assay revealed that these SNPs resulted in the increased promoter activity of dnt and conferred the increased ability to produce DNT on the bacteria. Similar or identical SNPs were also found in other pig isolates kept in our laboratory, all of which produce a larger amount of DNT than RB50. Our analysis revealed that substitution of at least two of the four bases, at positions -72 and +22, influenced the promoter activity for dnt. These results imply that these SNPs are involved in the pathogenicity of bordetellae specific to pig diseases.

Indexed as

Gene Expression Regulation, BacterialPolymorphism, Single NucleotideAnimalsBase SequenceBordetella bronchisepticaHumansMolecular Sequence DataPromoter Regions, GeneticSpecies SpecificityTransglutaminasesVirulence Factors, Bordetelladermonecrotic toxin, BordetellaTransglutaminasesVirulence Factors, Bordetella

Identifiers

PMID25642712
PMCPMC4314077
OpenAlexW1978463061

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.