ArticleACS macro letters2015
Biophysical Characterization of Human Protamine-1 as a Responsive CEST MR Contrast Agent.
Article in ACS macro letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Protamine-1 encoded recombinant adeno-associated virus for enhanced brain magnetic resonance imaging.Magnetic resonance letters · 2026Article
- Novel non‑metal‑based contrast agents for MR imaging: Emerging approaches and clinical perspectives (Review).International journal of oncology · 2025Review
- Development of a synthetic biosensor for chemical exchange MRI utilizing in silico optimized peptides.NMR in biomedicine · 2023Article
- Protein and peptide engineering for chemical exchange saturation transfer imaging in the age of synthetic biology.NMR in biomedicine · 2023Review
- Development of a Synthetic Biosensor for Chemical Exchange MRI UtilizingbioRxiv : the preprint server for biology · 2023Article
- Exploring Structures and Dynamics of Protamine Molecules through Molecular Dynamics Simulations.ACS omega · 2022Article
- Designing Protein-Based Probes for Sensing Biological Analytes with Magnetic Resonance Imaging.Analysis & sensing · 2022Article
- Brain pH Measurement Using AACID CEST MRI Incorporating the 2 ppm Amine Resonance.Tomography (Ann Arbor, Mich.) · 2022Article
- Redesigned reporter gene for improved proton exchange-based molecular MRI contrast.Scientific reports · 2020Article
- Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.Bioconjugate chemistry · 2020Review
- Nanomolar small-molecule detection using a genetically encodedChemical science · 2017Article
- Going Deeper: Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function.Biochemistry · 2017Review
- Cellular and Molecular Imaging Using Chemical Exchange Saturation Transfer.Topics in magnetic resonance imaging : TMRI · 2016Review
- Advances in Magnetic Resonance Imaging Contrast Agents for Biomarker Detection.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Review
- Chemical Exchange Saturation Transfer (CEST) Agents: Quantum Chemistry and MRI.Chemistry (Weinheim an der Bergstrasse, Germany) · 2016Article
- Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.Chemical communications (Cambridge, England) · 2015Article
- Double agents and secret agents: the emerging fields of exogenous chemical exchange saturation transfer and TResearch and reports in nuclear medicine · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The protamines are a low-molecular-weight, arginine-rich family of nuclear proteins that protect chromosomal DNA in germ cells by packing it densely using electrostatic interactions. Human protamine-1 (hPRM1) has been developed as a magnetic resonance imaging (MRI) chemical exchange saturation transfer (CEST) reporter gene, based on a sequence that is approximately 50% arginine, which has a side chain with rapidly exchanging protons. In this study, we have synthesized hPRM1 and determined how its CEST MRI contrast varies as a function of pH, phosphorylation state, and upon noncovalent interaction with nucleic acids and heparin (as antagonist). CEST contrast was found to be highly sensitive to phosphorylation on serine residues, intra- and intermolecular disulfide bridge formation, and the binding of negatively charged nucleotides and heparin. In addition, the nucleotide binding constants (
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.