ArticleMolecular cytogenetics2015
Surface-spreading technique of meiotic cells and immunodetection of synaptonemal complex proteins in teleostean fishes.
Article in Molecular cytogenetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- New insights into the putative XX/XY sex chromosomal system in blue-eyed red-fin pleco Hypostomus soniae (Siluriformes, Loricariidae).Journal of fish biology · 2026Article
- Meiotic Recombination in Teleosts.Advances in experimental medicine and biology · 2026Review
- A cyclical switch of gametogenic pathways in hybrids depends on the ploidy level.Communications biology · 2024Article
- Changes in Ploidy Drive Reproduction Transition and Genomic Diversity in a Polyploid Fish Complex.Molecular biology and evolution · 2022Article
- Kinetic Activity of Chromosomes and Expression of Recombination Genes in Achiasmatic Meiosis ofInternational journal of molecular sciences · 2022Article
- Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences inComparative cytogenetics · 2022Article
- Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids fromInternational journal of molecular sciences · 2021Article
- Meiotic self-pairing of the Psalidodon (Characiformes, Characidae) iso-B chromosome: A successful perpetuation mechanism.Genetics and molecular biology · 2021Article
- Parthenogenesis as a Solution to Hybrid Sterility: The Mechanistic Basis of Meiotic Distortions in Clonal and Sterile Hybrids.Genetics · 2020Article
- Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae).Comparative cytogenetics · 2016Article
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7 authors.
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Abstract
backgroundDifferent moderrn methodologies are presently available to analyze meiotic chromosomes. These methods permit investigation of the behavior of chromosomes in the normal complement and of sex and B chromosomes, two special types of chromosomes that are associated with the A complement and are present in many organisms, including fishes. However, meiotic studies are still scarce in fishes, considering the wide number of species in this group.. Here, we describe a new protocol for the visualization of the synaptonemal complex in spermatocytes and oocytes of fishes and to the sequential use of the technique with other procedures and techniques such as immunodetection of the synaptonemal complex protein with a specific antibody and co-detection of DNA sequences by FISH.
resultsThe meiotic surface-spreading protocol used in the present proposal worked well in representative species of four fish orders and was useful in obtaining good results even in small specimens. Fish-specific antibodies and commercial products worked similarly well to detect synaptonemal complex (SC) proteins. The sequential application of fluorescence in situ hybridization using specific probes showed clear signals associated with the SC structures identified by immunostaining.
conclusionHere, we provide a useful and applicable immunofluorescent protocol for the visualization of synaptonemal complex proteins in the meiotic cells of fishes in surface-spreading preparations. Furthermore, this technique allows for the sequential application of other cytogenetic procedures.
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