ArticleIranian journal of parasitology2014
Molecular Identification of Giardia duodenalis Isolates from Fars Province, Iran.
Article in Iranian journal of parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Prevalence and genotyping of Giardia duodenalis infections in humans in Thailand: a systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- Occurrence and assemblage distribution of Giardia Duodenalis in symptomatic and asymptomatic patients in southeastern Iran (2019-2022).Gut pathogens · 2024Article
- Molecular epidemiology and multilocus genotyping ofParasite epidemiology and control · 2024Article
- Genotyping ofArchives of Razi Institute · 2023Article
- Multilocus sequence typing ofFrontiers in medicine · 2022Article
- Parasites, Drugs and Captivity:Biology · 2021Article
- Isoenzyme profiles and phylogenetic analysis of Giardia duodenalis isolates from Iranian patients.Environmental science and pollution research international · 2020Article
- Report of Giardia assemblages and giardiasis in residents of Guilan province-Iran.Parasitology research · 2020Article
- Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.PloS one · 2020Article
- Article
- Spatial distribution ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Article
- Molecular genotyping of Giardia duodenalis in children from Behbahan, southwestern Iran.Parasitology research · 2018Article
- Molecular detection and identification ofVeterinary research forum : an international quarterly journal · 2018Article
- Subtype analysis of Giardia duodenalis isolates from municipal and domestic raw wastewaters in Iran.Environmental science and pollution research international · 2017Article
- Comparison and evaluation of four methods for extracting DNA fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- First genetic characterisation of Giardia in human isolates from Jordan.Parasitology research · 2016Article
- Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia.BMC microbiology · 2016Article
- Phylogenetic Analysis ofIranian journal of parasitologyArticle
- Genotype Characteristics ofIranian journal of parasitologyArticle
- CanIranian journal of parasitologyArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiardia duodenalis is one of the most common human intestinal protozoan parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. The present study aimed to identify the prevalence of G. duodenalis isolates and determine the most common of its assemblages in the patients referring to health centers and hospitals in Fars province, Iran that will be subjected to further molecular investigation.
methodsWe collected 1000 human fecal samples from health centers and hospitals in Shiraz, Iran in a one year period from September 2009 to August 2010. Microscopic examination for the presence of G. duodenalis cysts and trophozoites was performed by direct wet mount before and after the concentration techniques. Extraction of DNA was performed by Phenol-Chloroform-Isoamylalcohol (PCI). G. duodenalis-positive specimens were analyzed by PCR. A fragment of the SSU-rDNA (292 bp) gene was amplified by PCR using the forward primer RH11 and the reverse primer RH4. Genotyping was performed using sequence analysis of G. duodenalis glutamate dehydrogenase gene using primers GDHeF, GDHiF, and GDHiR.
resultsThe prevalence of Giardia infection was 10.7% (107/1000) examined based on microscopic examination. PCR identified 80% (40/50) of the samples as positive for G. duodenalis based on SSU-rDNA amplification on sucrose gradient samples. Besides, genotyping results indicated 32 isolates (80%) as assemblage AII and 8 isolates (20%) as assemblage BIII and BIV based on the DNA sequence analysis of the glutamate dehydrogenase locus of G. duodenalis.
conclusionThe findings of this study emphasize that Iran (Fars Province) is a favorable area for giardiasis with an anthroponotic infection route.
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25642262PMC4289883W2186578576What OpenQuestion holds
Registered trials
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