ReviewFrontiers in microbiology2014
Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi.
Review in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
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- Direct Cloning and Heterologous Expression of the Dmxorosin Biosynthetic Gene Cluster fromInternational journal of molecular sciences · 2025Article
- Multi-omics reveals the regulatory effects of Chinese herbal medicine substrates on secondary metabolite biosynthesis inFrontiers in microbiology · 2025Article
- Synergistic Biocontrol and Growth Promotion in Strawberries by Co-CulturedJournal of fungi (Basel, Switzerland) · 2024Article
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- Evolutionary investigations of the biosynthetic diversity in the skin microbiome usingMicrobial genomics · 2023Article
- Linking Lichen Metabolites to Genes: Emerging Concepts and Lessons from Molecular Biology and Metagenomics.Journal of fungi (Basel, Switzerland) · 2023Review
- Phytotoxic Metabolites Produced by Fungi Involved in Grapevine Trunk Diseases: Progress, Challenges, and Opportunities.Plants (Basel, Switzerland) · 2022Review
- Genomic Comparative Analysis ofMetabolites · 2022Article
- Fungal secondary metabolites in food and pharmaceuticals in the era of multi-omics.Applied microbiology and biotechnology · 2022Review
- Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synthaser: a CD-Search enabled Python toolkit for analysing domain architecture of fungal secondary metabolite megasynth(et)ases.Fungal biology and biotechnology · 2021Article
- Genomic Analyses ofJournal of fungi (Basel, Switzerland) · 2021Article
- Epigenetic Modifiers Revamp Secondary Metabolite Production in EndophyticFrontiers in microbiology · 2021Article
- cblaster: a remote search tool for rapid identification and visualization of homologous gene clusters.Bioinformatics advances · 2021Article
- Victorin, the host-selective cyclic peptide toxin from the oat pathogenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Melleins-Intriguing Natural Compounds.Biomolecules · 2020Review
- Comparative Genomic Analysis of Ochratoxin A Biosynthetic Cluster in Producing Fungi: New Evidence of a Cyclase Gene Involvement.Frontiers in microbiology · 2020Article
- Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Genomics has revolutionized the research on fungal secondary metabolite (SM) biosynthesis. To elucidate the molecular and enzymatic mechanisms underlying the biosynthesis of a specific SM compound, the important first step is often to find the genes that responsible for its synthesis. The accessibility to fungal genome sequences allows the bypass of the cumbersome traditional library construction and screening approach. The advance in next-generation sequencing (NGS) technologies have further improved the speed and reduced the cost of microbial genome sequencing in the past few years, which has accelerated the research in this field. Here, we will present an example work flow for identifying the gene cluster encoding the biosynthesis of SMs of interest using an NGS approach. We will also review the different strategies that can be employed to pinpoint the targeted gene clusters rapidly by giving several examples stemming from our work.
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