ArticleJournal of tissue engineering and regenerative medicine2017
Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line.
Article in Journal of tissue engineering and regenerative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Effects of PDMS culture on stem cell differentiation towards definitive endoderm and hepatocytes.Acta biomaterialia · 2025Article
- A Cell Culture Chip with Transparent, Micropillar-Decorated Bottom for Live Cell Imaging and Screening of Breast Cancer Cells.Micromachines · 2022Article
- Hepatitis B virus infection modeling using multi-cellular organoids derived from human induced pluripotent stem cells.World journal of gastroenterology · 2021Review
- A dual-ink 3D printing strategy to engineer pre-vascularized bone scaffolds in-vitro.Materials science & engineering. C, Materials for biological applications · 2021Article
- Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out.Nanomaterials (Basel, Switzerland) · 2021Article
- Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds.Journal of orthopaedic translation · 2021Article
- Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell-derived skeletal myocytes.Biotechnology and bioengineering · 2019Article
- Micro-Engineered Models of Development Using Induced Pluripotent Stem Cells.Frontiers in bioengineering and biotechnology · 2019Review
- Cytoskeletal tension regulates mesodermal spatial organization and subsequent vascular fate.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- In Vitro Microscale Models for Embryogenesis.Advanced biosystems · 2018Article
- Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence.Nature communications · 2017Article
- Designer Hydrogels for Precision Control of Oxygen Tension and Mechanical Properties.Journal of materials chemistry. B · 2015Article
- Stochasticity and Spatial Interaction Govern Stem Cell Differentiation Dynamics.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The multifaceted extracellular milieu presents biochemical and biophysical stimuli that influence stem cell differentiation. Two-dimensional (2D) micropatterned substrates allow the presentation of these cues in spatially defined geometries that have been demonstrated to guide stem cell fate decisions. Leveraging stem cells to reconstruct microvasculature, made up of an inner lining of endothelial cells (ECs) supported by pericytes, is critical to tissue-engineering advances; thus, methods to improve endothelial differentiation efficiency are vital to these efforts. In this study, we examine the hypothesis that the diameter of micropatterned islands influences endothelial differentiation from human induced pluripotent stem cells (hiPSCs). Comparing island diameters of 80, 140, 225 and 500 µm, we found that co-cultures of control ECs and pericytes did not yield variable ratios of cell types; however, when hiPSCs were differentiated toward a bicellular population of ECs and pericytes on these varying micropattern feature sizes, we found that smaller islands promoted EC differentiation efficiency, yielding a derived population composed of 70% ECs, which exhibited a greater sprouting propensity. Differentiation on the largest feature size exhibited a smaller EC yield, similar to that on non-patterned substrates. Taken together, these data demonstrate that micropatterned islands of varying diameters can be used to modulate EC differentiation efficiency. Copyright © 2015 John Wiley & Sons, Ltd.
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