ArticleNature communications2015
A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- Cell-Free DNA-Based Theranostics for Inflammatory Disorders.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- High-Viability Circulating Tumor Cells Sorting From Whole Blood at Single Cell Level Using Laser-Induced Forward Transfer-Assisted Microfiltration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Optimization of PCA Error Correction Conditions to Improve Efficiency of Virus Genome De Novo Synthesis.International journal of molecular sciences · 2024Article
- MOPSS: Toward high-fidelity oligonucleotides for clinical applications.Clinical and translational medicine · 2022Article
- High-throughputSTAR protocols · 2022Article
- Purification of multiplex oligonucleotide libraries by synthesis and selection.Nature biotechnology · 2022Article
- Barcode-free next-generation sequencing error validation for ultra-rare variant detection.Nature communications · 2019Article
- Synthetic biology advances and applications in the biotechnology industry: a perspective.Journal of industrial microbiology & biotechnology · 2018Article
- High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides.Nucleic acids research · 2018Article
- Highly selective retrieval of accurate DNA utilizing a pool of in situ-replicated DNA from multiple next-generation sequencing platforms.Nucleic acids research · 2018Article
- A systematic comparison of error correction enzymes by next-generation sequencing.Nucleic acids research · 2017Article
- A method for high-throughput production of sequence-verified DNA libraries and strain collections.Molecular systems biology · 2017Article
- In vivo high-throughput profiling of CRISPR-Cpf1 activity.Nature methods · 2017Article
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
- Toward a new paradigm of DNA writing using a massively parallel sequencing platform and degenerate oligonucleotide.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.