ArticleCurrent protocols in microbiology2015
Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms.
Article in Current protocols in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Genomic perplexity and the evolution of context-dependent function.Molecular biology and evolution · 2026Article
- Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle.Cell systems · 2025Article
- Chromosome architecture affects virulence and competitiveness inScience advances · 2025Article
- The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB): A Large-Scale Guide to Genetic Conditional Essentiality.Molecular microbiology · 2025Article
- A role for the S4-domain containing protein YlmH in ribosome-associated quality control in Bacillus subtilis.Nucleic acids research · 2024Article
- Restriction of arginine induces antibiotic tolerance in Staphylococcus aureus.Nature communications · 2024Article
- Coordinated adaptation ofmBio · 2024Article
- High-Throughput Mutant Screening via Transposon Sequencing.Cold Spring Harbor protocols · 2023Article
- Roles of RodZ and class A PBP1b in the assembly and regulation of the peripheral peptidoglycan elongasome in ovoid-shaped cells of Streptococcus pneumoniae D39.Molecular microbiology · 2022Article
- Activity-based annotation: the emergence of systems biochemistry.Trends in biochemical sciences · 2022Review
- A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance.Nature communications · 2022Article
- Development of the First Tractable Genetic System for Parvimonas micra, a Ubiquitous Pathobiont in Human Dysbiotic Disease.Microbiology spectrum · 2022Article
- Effect of diet on pathogen performance in the microbiome.Microbiome research reports · 2022Review
- Genetic Determinants in Salmonella enterica Serotype Typhimurium Required for OvercomingMicrobiology spectrum · 2021Article
- Transposon insertion site sequencing (TIS) of Pseudomonas aeruginosa.Journal of microbiology (Seoul, Korea) · 2021Article
- Bacterial factors required for Streptococcus pneumoniae coinfection with influenza A virus.Journal of biomedical science · 2021Article
- Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology.BMC biology · 2021Article
- Article
- Mycobacterial drug discovery.RSC medicinal chemistry · 2020Review
- Article
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3 authors.
Funding
Abstract
The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobial agents and vaccines. This unit presents Tn-seq, a method that has made it possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. Tn-seq can thus direct studies on the annotation of genes and untangle complex phenotypes. The method is based on the construction of a saturated transposon insertion library. After library selection, changes in the frequency of each insertion mutant are determined by sequencing flanking regions en masse. These changes are used to calculate each mutant's fitness. The method was originally developed for the Gram-positive bacterium Streptococcus pneumoniae, a causative agent of pneumonia and meningitis, but has now been applied to several different microbial species.
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