Evidence map›Paper›PMID 25640897›Full record

ArticleCurrent protocols in protein science2015

Ligand-directed profiling of organelles with internalizing phage libraries.

Andrey S Dobroff, Roberto Rangel, Liliana Guzman-Roja, Carolina C Salmeron, Juri G Gelovani, Richard L Sidman, Cristian G Bologa, Tudor I Oprea, C Jeffrey Brinker, Renata Pasqualini and 1 more

Abstract read
In one paragraph

Article in Current protocols in protein science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Andrey S DobroffDivision of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
Roberto RangelCancer Research Program, Houston Methodist Research Institute, Houston, Texas.
Liliana Guzman-RojaCancer Research Program, Houston Methodist Research Institute, Houston, Texas.
Carolina C SalmeronDivision of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
Juri G GelovaniDepartment of Biomedical Engineering, Wayne State University, Detroit, Michigan.
Richard L SidmanDepartment of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts.
Cristian G BologaTranslational Informatics Division, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
Tudor I OpreaTranslational Informatics Division, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
C Jeffrey BrinkerDepartment of Chemical and Nuclear Engineering, The University of New Mexico Cancer Center, Albuquerque, New Mexico.
Renata PasqualiniDivision of Molecular Medicine, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
Wadih ArapDivision of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.

Funding

Texas Center for Cancer NanomedicineU54CA151668 · NCI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI FERRARI, MAURO · 2010 to 2014
$12.4M
NCI NIH HHS U54 CA151668
6 · The paper itself

Abstract

Phage display is a resourceful tool to, in an unbiased manner, discover and characterize functional protein-protein interactions, create vaccines, and engineer peptides, antibodies, and other proteins as targeted diagnostic and/or therapeutic agents. Recently, our group has developed a new class of internalizing phage (iPhage) for ligand-directed targeting of organelles and to identify molecular pathways within live cells. This unique technology is suitable for applications ranging from fundamental cell biology to drug development. This unit describes the methods for generating and screening the iPhage display system, and explains how to select and validate candidate internalizing homing peptide.

Indexed as

Cell Line, TumorCell Surface Display TechniquesCloning, MolecularCytological TechniquesEscherichia coliHumansOrganellesintracellular receptorsintracellular targetingmammalian cellsorganellespenetratinpeptidesphage displayproteomics

Identifiers

PMID25640897
PMCPMC4339060

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.