ArticlePlant biotechnology journal2015
An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds.
Article in Plant biotechnology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Making fish oils in plants: from alpha to omega.The New phytologist · 2026Review
- Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.Plant physiology · 2025Review
- Identification of novel candidate genes for regulating oil composition in soybean seeds under environmental stresses.Frontiers in plant science · 2025Article
- Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster.Transgenic research · 2023Article
- Profiling Phospholipids within Atlantic SalmonMetabolites · 2022Article
- Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa.Plant biotechnology journal · 2022Article
- Multigene Transformation Through Cre-lox Mediated Site-Specific Integration in Rice.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina.Biotechnology for biofuels · 2021Article
- Recombinase-mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus.Plant direct · 2020Article
- Metabolic Engineering a Model OilseedFrontiers in plant science · 2020Review
- Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids.Biotechnology for biofuels · 2020Article
- Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants.Plant biotechnology journal · 2019Article
- Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway.Molecular biology reports · 2019Article
- Camelina sativa, an oilseed at the nexus between model system and commercial crop.Plant cell reports · 2018Review
- Seeds as oil factories.Plant reproduction · 2018Review
- Effect of level of soluble fiber and n-6/n-3 fatty acid ratio on performance of rabbit does and their litters.Journal of animal science · 2018Article
- Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds.Biotechnology letters · 2017Review
- Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa.Scientific reports · 2017Article
- Tailoring the composition of novel wax esters in the seeds of transgenic Camelina sativa through systematic metabolic engineering.Plant biotechnology journal · 2017Article
- Synthetic redesign of plant lipid metabolism.The Plant journal : for cell and molecular biology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The synthesis and accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa is demonstrated using the so-called alternative pathway. This aerobic pathway is found in a small number of taxonomically unrelated unicellular organisms and utilizes a C18 Δ9-elongase to generate C20 PUFAs. Here, we evaluated four different combinations of seed-specific transgene-derived activities to systematically determine the potential of this pathway to direct the synthesis of eicosapentaenoic acid (EPA) in transgenic plants. The accumulation of EPA and the related omega-3 LC-PUFA eicosatetraenoic acid (ETA) was observed up to 26.4% of total seed fatty acids, of which ETA was 9.5%. Seed oils such as these not only represent an additional source of EPA, but also an entirely new source of the bona fide fish oil ETA. Detailed lipidomic analysis of the alternative pathway in Camelina revealed that the acyl-substrate preferences of the different activities in the pathway can still generate a substrate-dichotomy bottleneck, largely due to inefficient acyl-exchange from phospholipids into the acyl-CoA pool. However, significant levels of EPA and ETA were detected in the triacylglycerols of transgenic seeds, confirming the channelling of these fatty acids into this storage lipid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.