ArticleTraffic (Copenhagen, Denmark)2015
Tagging strategies strongly affect the fate of overexpressed caveolin-1.
Article in Traffic (Copenhagen, Denmark), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Evolutionarily diverse caveolins share a common structural framework built around amphipathic disks.The Journal of cell biology · 2025Article
- Formation of intracellular vesicles within the GramMicrobial cell factories · 2022Article
- Caveolin1 Tyrosine-14 Phosphorylation: Role in Cellular Responsiveness to Mechanical Cues.The Journal of membrane biology · 2020Review
- Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy.Science advances · 2020Article
- The Na/K-ATPase α1 and c-Src form signaling complex under native condition: A crosslinking approach.Scientific reports · 2020Article
- Caveolin elastin-like polypeptide fusions mediate temperature-dependent assembly of caveolar microdomains.ACS biomaterials science & engineering · 2020Article
- Designing a rigorous microscopy experiment: Validating methods and avoiding bias.The Journal of cell biology · 2019Review
- Reciprocal regulation of eNOS and caveolin-1 functions in endothelial cells.Molecular biology of the cell · 2018Article
- Caveolins as Regulators of Stress Adaptation.Molecular pharmacology · 2018Review
- A disease-associated frameshift mutation in caveolin-1 disrupts caveolae formation and function through introduction of a de novo ER retention signal.Molecular biology of the cell · 2017Article
- Caveolins and cavins in the trafficking, maturation, and degradation of caveolae: implications for cell physiology.American journal of physiology. Cell physiology · 2017Review
- Caveolin-1 is an aggresome-inducing protein.Scientific reports · 2016Article
- Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy.Traffic (Copenhagen, Denmark) · 2016Article
- Caveolin proteins: a molecular insight into disease.Frontiers of medicine · 2016Review
- Activation of Endothelial Nitric Oxide (eNOS) Occurs through Different Membrane Domains in Endothelial Cells.PloS one · 2016Article
- Assembly and Turnover of Caveolae: What Do We Really Know?Frontiers in cell and developmental biology · 2016Review
- Unconserved C terminal of human cytomegalovirus tegument protein pUL76 elicits nuclear aggresome formation and induces DNA damage in transfected cells.Journal of biomedical science · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Caveolin-1 (Cav1) is the primary scaffolding protein of caveolae, flask-shaped invaginations of the plasma membrane thought to function in endocytosis, mechanotransduction, signaling and lipid homeostasis. A significant amount of our current knowledge about caveolins and caveolae is derived from studies of transiently overexpressed, C-terminally tagged caveolin proteins. However, how different tags affect the behavior of ectopically expressed Cav1 is still largely unknown. To address this question, we performed a comparative analysis of the subcellular distribution, oligomerization state and detergent resistance of transiently overexpressed Cav1 labeled with three different C-terminal tags (EGFP, mCherry and myc). We show that addition of fluorescent protein tags enhances the aggregation and/or degradation of both wild-type Cav1 and an oligomerization defective P132L mutant. Strikingly, complexes formed by overexpressed Cav1 fusion proteins excluded endogenous Cav1 and Cav2, and the properties of native caveolins were largely preserved even when abnormal aggregates were present in cells. These findings suggest that differences in tagging strategies may be a source of variation in previously published studies of Cav1 and that overexpressed Cav1 may exert functional effects outside of caveolae. They also highlight the need for a critical re-evaluation of current knowledge based on transient overexpression of tagged Cav1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.