ArticleMolecular genetics and genomics : MGG2015
Genetic redundancy is prevalent within the 6.7 Mb Sinorhizobium meliloti genome.
Article in Molecular genetics and genomics : MGG, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Deciphering gene redundancy in prokaryotic genomes provides evolutionary insights for pathogenicity and its roles in clinical infections.Nature communications · 2025Article
- What makes a megaplasmid?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Metabolic Modeling ofMicroorganisms · 2019Article
- Identification of Modules With Similar Gene Regulation and Metabolic Functions Based on Co-expression Data.Frontiers in molecular biosciences · 2019Article
- Absence of the Nitrous Oxide Reductase Gene Cluster in Commercial Alfalfa Inoculants Is Probably Due to the Extensive Loss of Genes During Rhizobial Domestication.Microbial ecology · 2018Article
- Inter-replicon Gene Flow Contributes to Transcriptional Integration in theG3 (Bethesda, Md.) · 2018Article
- Robustness encoded across essential and accessory replicons of the ecologically versatile bacterium Sinorhizobium meliloti.PLoS genetics · 2018Article
- Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes.Frontiers in microbiology · 2018Review
- Microevolution Rather than Large Genome Divergence Determines the Effectiveness of Legume-Rhizobia Symbiotic Interaction Under Field Conditions.Journal of molecular evolution · 2017Article
- PhoU Allows Rapid Adaptation to High Phosphate Concentrations by Modulating PstSCAB Transport Rate in Sinorhizobium meliloti.Journal of bacteriology · 2017Article
- The Divided Bacterial Genome: Structure, Function, and Evolution.Microbiology and molecular biology reviews : MMBR · 2017Review
- Trade, Diplomacy, and Warfare: The Quest for Elite Rhizobia Inoculant Strains.Frontiers in microbiology · 2017Article
- Metabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti.Nature communications · 2016Article
- L-Hydroxyproline and d-Proline Catabolism in Sinorhizobium meliloti.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Biological pathways are frequently identified via a genetic loss-of-function approach. While this approach has proven to be powerful, it is imperfect as illustrated by well-studied pathways continuing to have missing steps. One potential limiting factor is the masking of phenotypes through genetic redundancy. The prevalence of genetic redundancy in bacterial species has received little attention, although isolated examples of functionally redundant gene pairs exist. Here, we made use of a strain of Sinorhizobium meliloti whose genome was reduced by 45 % through the complete removal of a megaplasmid and a chromid (3 Mb of the 6.7 Mb genome was removed) to begin quantifying the level of genetic redundancy within a large bacterial genome. A mutagenesis of the strain with the reduced genome identified a set of transposon insertions precluding growth of this strain on minimal medium. Transfer of these mutations to the wild-type background revealed that 10-15 % of these chromosomal mutations were located within duplicated genes, as they did not prevent growth of cells with the full genome. The functionally redundant genes were involved in a variety of metabolic pathways, including central carbon metabolism, transport, and amino acid biosynthesis. These results indicate that genetic redundancy may be prevalent within large bacterial genomes. Failing to account for redundantly encoded functions in loss-of-function studies will impair our understanding of a broad range of biological processes and limit our ability to use synthetic biology in the construction of designer cell factories.
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