Evidence map›Paper›PMID 25637681›Full record

ArticleAnalytical biochemistry2015

Applicability of fluorescence-based sensors to the determination of kinetic parameters for O₂ in oxygenases.

Natali V Di Russo, Steven D Bruner, Adrian E Roitberg

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Natali V Di RussoDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA. Electronic address: ndirusso@ufl.edu.
Steven D BrunerDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Adrian E RoitbergDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
University of Florida · US

Funding

Mechanisms of nonribosomal peptide natural product biosynthesisR01GM086570 · NIGMS · UNIVERSITY OF FLORIDA · PI BRUNER, STEVEN D · 2009 to 2013
$1.4M
Howard Hughes Medical InstituteNIGMS NIH HHS GM086570NIGMS NIH HHS R01 GM086570
6 · The paper itself

Abstract

Optical methods for O2 determination based on dynamic fluorescence quenching have been applied to measure oxygen uptake rates in cell culture and to determine intracellular oxygen levels. Here we demonstrate the applicability of fluorescence-based probes in determining kinetic parameters for O2 using as an example catalysis by a cofactor-independent oxygenase (DpgC). Fluorescence-based sensors provide a direct assessment of enzyme-catalyzed O2 consumption using commercially available, low-cost instrumentation that is easily customizable and, thus, constitutes a convenient alternative to the widely used Clark-type electrode, especially in cases where chemical interference is expected to be problematic.

Indexed as

ElectrodesFluorescent DyesKineticsOxygenOxygenasesFluorescent DyesOxygenOxygenasesEnzyme assaysEnzyme kineticsFluorescence quenchingOxygenOxygenases

Identifiers

PMID25637681
PMCPMC4402703
OpenAlexW2050297535

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.